BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K1962015
1
BBa_K1962015
A device for expression of Im-Ia in response to pH
2016-10-11T11:00:00Z
2016-10-12T03:50:34Z
Existing and new biobricks were combined using the RFC[10] standard.
This is a composite part for the production of the Immunity Protein to Colicin Ia in response to environmental pH changes. The composite part consists of a pH sensitive promoter (<i>asr</i> from biobrick <partinfo>BBa_K1231000</partinfo>, an RBS (<partinfo>BBa_B0030</partinfo>) and the Immunity Protein required to neutralise the bacteriocin activity of Colicin Ia (<partinfo>BBa_K1962001</partinfo>).
false
false
_2429_
8083
8083
9
false
RFC[10] compliant.
false
Frank Sargent
component2493641
1
BBa_K1231000
component2493643
1
BBa_B0030
component2493646
1
BBa_K1962001
annotation2493643
1
BBa_B0030
range2493643
1
149
163
annotation2493646
1
BBa_K1962001
range2493646
1
170
502
annotation2493641
1
BBa_K1231000
range2493641
1
1
140
BBa_K1962001
1
BBa_K1962001
Immunity Protein Im-Ia
2016-10-10T11:00:00Z
2016-10-11T03:07:20Z
This part was designed by back-translation of the primary amino acid sequence into DNA sequence, followed by optimisiation for expression in an E. coli chassis. The biobrick was then synthesised de novo by IDT.
This biobrick codes for the Immunity Protein for Colicin Ia (<partinfo>BBa_K1962000</partinfo>). Colicin Ia is a channel-forming bacteriocin that depolarizes the cytoplasmic inner membrane of target bacteria, leading to dissipation of cellular energy. This Immunity Protein is tightly linked to its specific Colicin bacteriocin domain to protect the colicinogenic cell from the cytotoxic activity of the colicin.
false
false
_2429_
8083
8083
9
false
Compliant with RFC[10].
false
Frank Sargent
annotation2491784
1
Immunity Protein Im-Ia
range2491784
1
1
333
BBa_K1231000
1
BBa_K1231000
The asr promoter is a pH-responsive promoter.
2013-09-12T11:00:00Z
2015-05-08T01:09:44Z
E. coli
This part contains the asr promoter with its native RBS. The asr promoter is a pH-responsive promoter native to E. coli. It induces transcription in acidic conditions (~pH 5.5).
false
false
_1545_
0
18773
9
In stock
false
None.
false
Viral Patel
BBa_K1962015_sequence
1
cgctgtaatttattcagcgtttgtacatatcgttacacgctgaaaccaaccactcacggaagtctgccattcccagggatatagttatttcaacggccccgcagtggggttaaatgaaaaaacaaattgagggtatgacatactagagattaaagaggagaaatactagatgaaccgtaaatactacttcaacaacatgtggtggggttgggttaccggtggttacatgctgtacatgtcttgggactacgagttcaaataccgtctgctgttctggtgcatctctctgtgcggtatggttctgtacccggttgcgaaatggtacatcgaagacaccgcgctgaaattcacccgtccggacttctggaactctggtttcttcgcggacaccccgggtaaaatgggtctgctggcggtttacaccggtaccgttttcatcctgtctctgccgctgtctatgatctacatcctgtctgttatcatcaaacgtctgtctgttcgt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1962001_sequence
1
atgaaccgtaaatactacttcaacaacatgtggtggggttgggttaccggtggttacatgctgtacatgtcttgggactacgagttcaaataccgtctgctgttctggtgcatctctctgtgcggtatggttctgtacccggttgcgaaatggtacatcgaagacaccgcgctgaaattcacccgtccggacttctggaactctggtttcttcgcggacaccccgggtaaaatgggtctgctggcggtttacaccggtaccgttttcatcctgtctctgccgctgtctatgatctacatcctgtctgttatcatcaaacgtctgtctgttcgt
BBa_K1231000_sequence
1
cgctgtaatttattcagcgtttgtacatatcgttacacgctgaaaccaaccactcacggaagtctgccattcccagggatatagttatttcaacggccccgcagtggggttaaatgaaaaaacaaattgagggtatgaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z