BBa_K1963004
1
BBa_K1963004
An sRNA system for down-regulation of E. coli fliC encoding flagellin.
2016-10-06T11:00:00Z
2016-10-23T09:17:38Z
This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.
This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.
false
false
_2430_
8083
8083
9
true
This part is RFC[10] compliant.
false
Frank Sargent
annotation2487508
1
sRNA
range2487508
1
194
218
annotation2487509
1
micC loop
range2487509
1
219
296
annotation2487507
1
proD promoter
range2487507
1
1
193
annotation2487510
1
terminator
range2487510
1
297
426
BBa_K1963004_sequence
1
cacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtataatatattcagggagaccacaacggtttccctctacaaataattttgtttaacttttaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcgttggtattaatgacttgtgccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggctttttttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z