BBa_K1964001
1
BBa_K1964001
Codon optimized GcABC-G1 for Saccharomyces cerevisiae
2016-10-13T11:00:00Z
2016-10-14T01:30:26Z
The original gene has been found in the genome of Grossmannia clavigera.
The submitted sequence was synthesized by idtDNA and codon optimized for Saccharomyces cerevisiae.
This part consists of a codon optimized version of GcABC-G1 for Saccharomyces cerevisiae. GcABC-G1 is a monoterpenoid efflux pump from Grossmannia clavigera, which mediates a higher resistance towards Limonene and in general monoterpenoids.
The efflux pump is part of the ABC-transporter family and leads to the transfer of monoterpenoids out of the organism into the surrounding enviroment.
In the project of the Marburger iGEM-Team 2016 the transporter was used to not only confer a higher resistance towards limonene in S. cerevisiae, but also to secrete the monoterpenoid and final product of the artificial endosymbiotic production line, perillyl alcohol, into the medium.
false
false
_2431_
29804
29804
9
false
Biobrick cutsites had to be codon optimized for Saccharomyces cerevisiae and the biobrick cutsites had to be removed during sequencing.
false
Steffen L??tke
BBa_K1964001_sequence
1
atggaaacagatagcaagtctgtggaaagcggggagaccgccgctatgccaggacaacagcagatcagttctaatgcacaagggcttatacacgcatatagcatggaactggtgagatcgtcctccagggcgacgggcggaggtggagcccccggacggaatccgttcacggggacatcgaacgacccggcgcttgatccacattctaaagccttcgatgccagaagatgggcccaggccgttctgcactcaacaggtgaaggacctgatcactgtccccgcccgaccgcgggagtggcatataggaacctgagggtacacggttacgggtcgcctactgactaccagaaagacgtttttaacgtactcttacaggcaccattggaagctgcacagtacttcatgagttcccgccgtggaagggaagtgcccattttgagagacgggtttgacggattggtcaggtcaggtgagatgcttttagtcttagggcgaccaggttccggggtgacgactctattgaagactgtcgcgggtgaaacaaatggtttacaggtcgacgcagaggcgttcataagttaccaaggcatacccatgcaagccattcaaaaacgtttccgcggggaagtagtctaccaggcggagacagacgtgcatttcccacagcttacagtagggcagacccttttgtttgcagccaaagctaggacaccacagatgcggcctgacggtgtaacacgcgcacaatatgcaaagcatatacgtgatgttgtgatggcggtattcggaatctcgcacacggttaacacaagggtagggtcggacctggtgcgaggtgtgtcaggtggagaaagaaagcgagtaagcattgccgaggtcgctctttctggatctgccttgcaatgttgggataattctacgcggggcttagattcggcaagtgctttgtccttcgcaaacactttaagactgagcactgaacttgcgggcactaccgcattagttgctatgtatcaggcgtcagaagctgcgtatgagacattcggaaaagtgtgtctactttacgagggccgacaaattttcttcggtcccgccaacgaggccaaggcgttcttcgtagacatgggctacgaatgcccggatagacagaccacagcagatttcttgacgtcactcacgaatccaggagaacgtgtagttcggcccggtttcgaaaaccgggttccgaggactcccgatgactttgtagcttattggaaagcaagtgcaacgcgcgcttccctactccaggatatagcggagttcgaccaggaacacccaatggatggaacgccaatagaggcaatggcgaccgtacgcaaagcccatcaggcacctttaacgccgaataaatctccgtttaccctatcatttcctcaacaggttgctttatgcatgacacgcggttacgaacgcactatgggagacaagacttttttcattgtcacagttgggggtaatcttgtgatatctttagtccttggatcggttttctaccaattgtcacccgatgcgagctccatcacgagccggtgtattctcttattctttgcgattctttttaatgctctctcatcgagcttagagatactttcgttgtatgctcaacggcccatagttgagaagcatgcacgatacgcgttatatactccttcggcagaggccgtctcatctgccttttgcgaactaccgagtaaaatattttcagcgattgccttcaatattcctctctatttcatggccgacttgaggcatggggccggtcatttcttttttttcttgctatttgcctttacgtgcaccctaactatgtcgttcatcctgcgtacgattgggcaagcctcgcgaactgtgcaggaggccttgacccctgctgccgtgtttattatatcgctcgtgatctatactgggttcgttattccagtcaaatcgatgcaaggctggatgcgctggataaactatctaaacccgatcgcctatgcttacgaatctttgctcgtcaatgaactgtctggccggaattttccttgtgcatcgtttgtaccggcataccccaatctatccagtagtgagcatacttgtagtacagccggggcggctcccggcgcagattttgtggttggggacaccatcttgaacagtagctatgagtactaccacgctcataaatggcggaatttgggtatattaattggtttcttaatagcgttctttttcgcttaccttgtggcaagtgaatatatcacggccgaacaatctaagggcgaagttctcgttttccgtcgagggcacaaagagtccgctgtcgtagaaaggaaaaccgccacgtctgacgactcagacggcgagaaaggacaccagacggagcaaaaggatatttgtcattggagaaacgtgtgttatgatatcaccatcaagggccagggaaggcgtctcttagatcacgtcgacggatgggtgaagccgggtacgttaacgtgtctgatgggagtttcgggggcaggaaaaaccacactgctggacgtgttggcgaaccgcgtaaccatgggcgtagtaacaggtgacatgctcgtgaatgggagcccgcgtgacagtagttttcaacggaaaactggttatgtacagcaacaagatgtccatttagagacaagtactgtgagagaagcgctgagattctccgctcagcttcgccagccaactacggttagtacacaagacaaatacatcttcgtggaagaggtaatagaattattagagatggacgagtacgcagatgcaatagttggagtaccgggcacgggcttaaacgtcgagcaacgaaagaggctaactattggcgtggagctggcagctaaaccagacttactgctatttcttgacgagcctacgtcaggtctcgactcccagactgcctggagcgtagcagcgctcattcgtaagttatcagctcgcgggcaggcggtactatgtactatccatcagccttcagccttactgtaccaacaatttgataggattcttttattagccgccgggggaaggacggtctatttcggggatatcggtcccaatgcagaaaccattataagttatttcgagcggaatggggccgagccctgtgggcaagacgaaaatccggccgagtggatgctgtcagttatcggagctgggccaggtggggtcgcgaagcaagactgggtatcgatttggcgtaatagtgacgagtacagcgctgtgcaagctgaattagataaccttgccaaacgcaaggatacgatggcgtcttcaggtgcgaccgacgcagccgcagtgactacatatgccacgccgtttttcttccagctctacatgtgctcaaagagagtgttcgaacagtactggcgtactccttcctacatatacgctaagatgattctctgtttcgcggtatcgctgtttataggtttatcgtttagaaaagcgccattgtcggagcagggactacagaaccaaatgtttagtatattcatgctactcgtgatcttcgcgtttctagcttatcagacgatgccccacttcattcgtcaacgagagctgtacgaaatacgtgaacgcgcatcacgtacatactcctggtatgttttcatgttggctaatattatcgttgaattaccctggaatacgatagcctcgcttttagtcttcctccccttctactacatagtgggcatgaatcataatgccgaagcgacccactctgtaagtgaacgaggaggactcatgttcctattagtctgggtgtttttggtattcgaatctacgtttacggatatggttgtcgccggaagtccaacagccgaacttggggccacgatggccctactcctatttgcttttacattaattttttgtggcgtgatggtaggtaaagaccaattaccgggtttttggatatttatgtatcgcgtgtcgcctcttacatacttggtcggcggattactggcgacaggggtgggtcatcatgaagtgacctgcactgcccgggagctccttagcttccagcctgtaggtaatcagacttgtctcgaatacatgaccccatacatgaagttggccggaggtaaggttattaatccaaatgcggtggcaccggcttcgtgcgagttctgcactctggcaaacacagatgcatttctagctagtattaacgtatcatatgatcaaaggtggcgagactttggcttaatgtgggcgtacgtagtgttcaatgtcttcggcgctctcttcatgtattggctggtccgtgcacccaagggcgacttaaaggcgaggttatttaaattggtcgggaaaacagca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z