BBa_K1964001 1 BBa_K1964001 Codon optimized GcABC-G1 for Saccharomyces cerevisiae 2016-10-13T11:00:00Z 2016-10-14T01:30:26Z The original gene has been found in the genome of Grossmannia clavigera. The submitted sequence was synthesized by idtDNA and codon optimized for Saccharomyces cerevisiae. This part consists of a codon optimized version of GcABC-G1 for Saccharomyces cerevisiae. GcABC-G1 is a monoterpenoid efflux pump from Grossmannia clavigera, which mediates a higher resistance towards Limonene and in general monoterpenoids. The efflux pump is part of the ABC-transporter family and leads to the transfer of monoterpenoids out of the organism into the surrounding enviroment. In the project of the Marburger iGEM-Team 2016 the transporter was used to not only confer a higher resistance towards limonene in S. cerevisiae, but also to secrete the monoterpenoid and final product of the artificial endosymbiotic production line, perillyl alcohol, into the medium. false false _2431_ 29804 29804 9 false Biobrick cutsites had to be codon optimized for Saccharomyces cerevisiae and the biobrick cutsites had to be removed during sequencing. false Steffen L??tke BBa_K1964001_sequence 1 atggaaacagatagcaagtctgtggaaagcggggagaccgccgctatgccaggacaacagcagatcagttctaatgcacaagggcttatacacgcatatagcatggaactggtgagatcgtcctccagggcgacgggcggaggtggagcccccggacggaatccgttcacggggacatcgaacgacccggcgcttgatccacattctaaagccttcgatgccagaagatgggcccaggccgttctgcactcaacaggtgaaggacctgatcactgtccccgcccgaccgcgggagtggcatataggaacctgagggtacacggttacgggtcgcctactgactaccagaaagacgtttttaacgtactcttacaggcaccattggaagctgcacagtacttcatgagttcccgccgtggaagggaagtgcccattttgagagacgggtttgacggattggtcaggtcaggtgagatgcttttagtcttagggcgaccaggttccggggtgacgactctattgaagactgtcgcgggtgaaacaaatggtttacaggtcgacgcagaggcgttcataagttaccaaggcatacccatgcaagccattcaaaaacgtttccgcggggaagtagtctaccaggcggagacagacgtgcatttcccacagcttacagtagggcagacccttttgtttgcagccaaagctaggacaccacagatgcggcctgacggtgtaacacgcgcacaatatgcaaagcatatacgtgatgttgtgatggcggtattcggaatctcgcacacggttaacacaagggtagggtcggacctggtgcgaggtgtgtcaggtggagaaagaaagcgagtaagcattgccgaggtcgctctttctggatctgccttgcaatgttgggataattctacgcggggcttagattcggcaagtgctttgtccttcgcaaacactttaagactgagcactgaacttgcgggcactaccgcattagttgctatgtatcaggcgtcagaagctgcgtatgagacattcggaaaagtgtgtctactttacgagggccgacaaattttcttcggtcccgccaacgaggccaaggcgttcttcgtagacatgggctacgaatgcccggatagacagaccacagcagatttcttgacgtcactcacgaatccaggagaacgtgtagttcggcccggtttcgaaaaccgggttccgaggactcccgatgactttgtagcttattggaaagcaagtgcaacgcgcgcttccctactccaggatatagcggagttcgaccaggaacacccaatggatggaacgccaatagaggcaatggcgaccgtacgcaaagcccatcaggcacctttaacgccgaataaatctccgtttaccctatcatttcctcaacaggttgctttatgcatgacacgcggttacgaacgcactatgggagacaagacttttttcattgtcacagttgggggtaatcttgtgatatctttagtccttggatcggttttctaccaattgtcacccgatgcgagctccatcacgagccggtgtattctcttattctttgcgattctttttaatgctctctcatcgagcttagagatactttcgttgtatgctcaacggcccatagttgagaagcatgcacgatacgcgttatatactccttcggcagaggccgtctcatctgccttttgcgaactaccgagtaaaatattttcagcgattgccttcaatattcctctctatttcatggccgacttgaggcatggggccggtcatttcttttttttcttgctatttgcctttacgtgcaccctaactatgtcgttcatcctgcgtacgattgggcaagcctcgcgaactgtgcaggaggccttgacccctgctgccgtgtttattatatcgctcgtgatctatactgggttcgttattccagtcaaatcgatgcaaggctggatgcgctggataaactatctaaacccgatcgcctatgcttacgaatctttgctcgtcaatgaactgtctggccggaattttccttgtgcatcgtttgtaccggcataccccaatctatccagtagtgagcatacttgtagtacagccggggcggctcccggcgcagattttgtggttggggacaccatcttgaacagtagctatgagtactaccacgctcataaatggcggaatttgggtatattaattggtttcttaatagcgttctttttcgcttaccttgtggcaagtgaatatatcacggccgaacaatctaagggcgaagttctcgttttccgtcgagggcacaaagagtccgctgtcgtagaaaggaaaaccgccacgtctgacgactcagacggcgagaaaggacaccagacggagcaaaaggatatttgtcattggagaaacgtgtgttatgatatcaccatcaagggccagggaaggcgtctcttagatcacgtcgacggatgggtgaagccgggtacgttaacgtgtctgatgggagtttcgggggcaggaaaaaccacactgctggacgtgttggcgaaccgcgtaaccatgggcgtagtaacaggtgacatgctcgtgaatgggagcccgcgtgacagtagttttcaacggaaaactggttatgtacagcaacaagatgtccatttagagacaagtactgtgagagaagcgctgagattctccgctcagcttcgccagccaactacggttagtacacaagacaaatacatcttcgtggaagaggtaatagaattattagagatggacgagtacgcagatgcaatagttggagtaccgggcacgggcttaaacgtcgagcaacgaaagaggctaactattggcgtggagctggcagctaaaccagacttactgctatttcttgacgagcctacgtcaggtctcgactcccagactgcctggagcgtagcagcgctcattcgtaagttatcagctcgcgggcaggcggtactatgtactatccatcagccttcagccttactgtaccaacaatttgataggattcttttattagccgccgggggaaggacggtctatttcggggatatcggtcccaatgcagaaaccattataagttatttcgagcggaatggggccgagccctgtgggcaagacgaaaatccggccgagtggatgctgtcagttatcggagctgggccaggtggggtcgcgaagcaagactgggtatcgatttggcgtaatagtgacgagtacagcgctgtgcaagctgaattagataaccttgccaaacgcaaggatacgatggcgtcttcaggtgcgaccgacgcagccgcagtgactacatatgccacgccgtttttcttccagctctacatgtgctcaaagagagtgttcgaacagtactggcgtactccttcctacatatacgctaagatgattctctgtttcgcggtatcgctgtttataggtttatcgtttagaaaagcgccattgtcggagcagggactacagaaccaaatgtttagtatattcatgctactcgtgatcttcgcgtttctagcttatcagacgatgccccacttcattcgtcaacgagagctgtacgaaatacgtgaacgcgcatcacgtacatactcctggtatgttttcatgttggctaatattatcgttgaattaccctggaatacgatagcctcgcttttagtcttcctccccttctactacatagtgggcatgaatcataatgccgaagcgacccactctgtaagtgaacgaggaggactcatgttcctattagtctgggtgtttttggtattcgaatctacgtttacggatatggttgtcgccggaagtccaacagccgaacttggggccacgatggccctactcctatttgcttttacattaattttttgtggcgtgatggtaggtaaagaccaattaccgggtttttggatatttatgtatcgcgtgtcgcctcttacatacttggtcggcggattactggcgacaggggtgggtcatcatgaagtgacctgcactgcccgggagctccttagcttccagcctgtaggtaatcagacttgtctcgaatacatgaccccatacatgaagttggccggaggtaaggttattaatccaaatgcggtggcaccggcttcgtgcgagttctgcactctggcaaacacagatgcatttctagctagtattaacgtatcatatgatcaaaggtggcgagactttggcttaatgtgggcgtacgtagtgttcaatgtcttcggcgctctcttcatgtattggctggtccgtgcacccaagggcgacttaaaggcgaggttatttaaattggtcgggaaaacagca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z