BBa_K1965008
1
BBa_K1965008
His:CIBN:cLuc:HA
2016-10-16T11:00:00Z
2016-10-19T02:56:33Z
Arabidopsis thali and Photinus pyralis (Common eastern firefly)
This part is one of the two components of the dimerazing system which is induced by blue light. We adapted this system for the reconstitution of split luciferase to create a blue-light sensor. To the C-terminus of CIBN, C-terminus of split firefly luciferase was added. Upon induction with light and concequent dimerisation, the split luciferase is reconstituted and regains activity, which results in biolumiescence if luciferin is added.
false
false
_2432_
26210
30084
9
false
/
false
Nik Franko
annotation2515499
1
HA
range2515499
1
751
777
annotation2515487
1
CIBN
range2515487
1
22
528
annotation2515498
1
GSS linker
range2515498
1
742
750
annotation2515485
1
start
range2515485
1
1
3
annotation2515500
1
stop
range2515500
1
778
780
annotation2515497
1
cLuc
range2515497
1
559
741
annotation2515496
1
10 GS linker
range2515496
1
529
558
annotation2515486
1
His
range2515486
1
4
21
BBa_K1965008_sequence
1
atgcaccaccaccaccaccacaatggagctataggaggtgaccttttgctcaattttcctgacatgtcggtcctagagcgccaaagggctcacctcaagtacctcaatcccacctttgattctcctctcgccggcttctttgccgattcttcaatgattaccggcggcgagatggacagctatctttcgactgccggtttgaatcttccgatgatgtacggtgagacgacggtggaaggtgattcaagactctcaatttcgccggaaacgacgcttgggactggaaatttcaaggcagcgaagtttgatacagagactaaggattgtaatgaggcggcgaagaagatgacgatgaacagagatgacctagtagaagaaggagaagaagagaagtcgaaaataacagagcaaaacaatgggagcacaaaaagcatcaagaagatgaaacacaaagccaagaaagaagagaacaatttctctaatgattcatctaaagtgacgaaggaattggagaaaacggattatattggcggctctggcggcggctccggaggctctaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgaatagtggttctggatacccatacgatgttccagattacgcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z