BBa_K1965018 1 BBa_K1965018 CaM(E31Q,E104Q):cLuc 2016-10-16T11:00:00Z 2016-10-17T04:53:51Z Photinus pyralis (Common eastern firefly) and Homo sapiens (Human) CaM(E31Q,E104Q):cLuc is a genetic fusion of human calmodulin with two introduced mutation E31Q, E104Q and C terminal fragment of split firefly luciferase. Both mutations are located in EF hands of calmodulin and lowers the construct's sensitivity to calcium ion concentration. The part in combination with BBa_K1965014 (nLuc:M13) reconstitutes luciferase activity upon calcium influx into the cell. false false _2432_ 30084 30084 9 false / false Nik Franko annotation2515552 1 CaM(E31Q,E104Q) range2515552 1 4 444 annotation2515565 1 cLuc range2515565 1 475 657 annotation2515551 1 start range2515551 1 1 3 annotation2515553 1 10GS linker range2515553 1 445 474 annotation2515620 1 GSG linker range2515620 1 658 666 annotation2515639 1 stop range2515639 1 694 696 annotation2515621 1 HA range2515621 1 667 693 BBa_K1965018_sequence 1 atggaccaactgacagaagagcagattgcagagttcaaagaagccttctcattattcgacaaggatggggacggcaccatcaccacaaagcagcttggcaccgttatgaggtcgcttggacaaaacccaacggaagcagaattgcaggatatgatcaatgaagtcgatgctgatggcaatggaacgatttactttcctgaatttcttactatgatggctagaaaaatgaaggacacagacagcgaagaggaaatccgagaagcattccgtgtttttgacaaggatgggaacggctatatcagtgctgctcagttacgtcatgtgatgacaaaccttggggagaagttaacagatgaagaagttgatgaaatgataagggaagcagatattgatggagatggccaagtaaactatgaagagtttgtacaaatgatgacagcaaagggcggctctggcggcggctccggaggctctaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgaatagtggttctggatacccatacgatgttccagattacgcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z