BBa_K1965018
1
BBa_K1965018
CaM(E31Q,E104Q):cLuc
2016-10-16T11:00:00Z
2016-10-17T04:53:51Z
Photinus pyralis (Common eastern firefly) and Homo sapiens (Human)
CaM(E31Q,E104Q):cLuc is a genetic fusion of human calmodulin with two introduced mutation E31Q, E104Q and C terminal fragment of split firefly luciferase. Both mutations are located in EF hands of calmodulin and lowers the construct's sensitivity to calcium ion concentration. The part in combination with BBa_K1965014 (nLuc:M13) reconstitutes luciferase activity upon calcium influx into the cell.
false
false
_2432_
30084
30084
9
false
/
false
Nik Franko
annotation2515552
1
CaM(E31Q,E104Q)
range2515552
1
4
444
annotation2515565
1
cLuc
range2515565
1
475
657
annotation2515551
1
start
range2515551
1
1
3
annotation2515553
1
10GS linker
range2515553
1
445
474
annotation2515620
1
GSG linker
range2515620
1
658
666
annotation2515639
1
stop
range2515639
1
694
696
annotation2515621
1
HA
range2515621
1
667
693
BBa_K1965018_sequence
1
atggaccaactgacagaagagcagattgcagagttcaaagaagccttctcattattcgacaaggatggggacggcaccatcaccacaaagcagcttggcaccgttatgaggtcgcttggacaaaacccaacggaagcagaattgcaggatatgatcaatgaagtcgatgctgatggcaatggaacgatttactttcctgaatttcttactatgatggctagaaaaatgaaggacacagacagcgaagaggaaatccgagaagcattccgtgtttttgacaaggatgggaacggctatatcagtgctgctcagttacgtcatgtgatgacaaaccttggggagaagttaacagatgaagaagttgatgaaatgataagggaagcagatattgatggagatggccaagtaaactatgaagagtttgtacaaatgatgacagcaaagggcggctctggcggcggctccggaggctctaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgaatagtggttctggatacccatacgatgttccagattacgcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z