LOCUS       BBa_K1965022            1800 bp    DNA     linear       23-Dec-2024
DEFINITION  nLuc:AP4:TEVs:P3mS-2A
ACCESSION   BBa_K1965022
VERSION     BBa_K1965022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     misc_feature    4..42
                     /label=cMyc-tag
     CDS             43..1512
                     /label=nLuc
     CDS             1543..1641
                     /label=AP4
     misc_feature    1600..1680
                     /label=TEV cleavage site
     CDS             1699..1797
                     /label=P3mS-2A
     misc_feature    1798..1800
                     /label=stop
ORIGIN
        1 atggagcaga agctgatcag cgaggaggac ctgggttctg gagaagatgc caaaaacatt
       61 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      121 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      181 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      241 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      301 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      361 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      421 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      481 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      541 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      601 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      661 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      721 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      781 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      841 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      901 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
      961 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1021 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1081 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1141 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1201 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1261 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1321 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1381 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1441 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1501 gaacacggta aaggcggctc tggcggcggc tccggaggct cttcacccga ggacgagctt
     1561 gctgctaatg aagaggagtt gcagcaaaat gaacaaaagt tggctcaaat taagcaaaaa
     1621 cttcaagcta tcaaatacgg tggcggctct ggcggcggcg aaaatttgta tttccagagt
     1681 ggcggctccg gaggctcttc ccccgaggac gagatccagc agctcgagga ggaggcctcc
     1741 caggccgagc agaagaactc ccagctgaag gagaagaacc agcagctgaa gtacggctaa
//