LOCUS       BBa_K1965037            2325 bp    DNA     linear       23-Nov-2024
DEFINITION  cycLuc_TEVs
ACCESSION   BBa_K1965037
VERSION     BBa_K1965037.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     misc_feature    4..21
                     /label=His
     CDS             22..138
                     /label=IntC
     misc_feature    139..156
                     /label=AU1
     CDS             157..1089
                     /label=cLuc
     misc_feature    1090..1122
                     /label=TEVp cleavage site
     CDS             1123..1812
                     /label=nLuc
     CDS             1813..2127
                     /label=IntN
     misc_feature    2128..2322
                     /label=PEST degradation tag
     misc_feature    2323..2325
                     /label=stop
ORIGIN
        1 atgcaccacc accaccacca catgatcaaa atagccacac gtaaatattt aggcaaacaa
       61 aatgtctatg acattggagt tgagcgcgac cataattttg cactcaaaaa tggcttcata
      121 gcttctaatt gtttcaatga tacttataga tatattgaca ccgctatcct cagcgtggtg
      181 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
      241 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
      301 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
      361 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
      421 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
      481 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
      541 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
      601 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
      661 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
      721 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
      781 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
      841 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
      901 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
      961 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     1021 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     1081 gccaagaagg gatccgaaaa tctctatttc cagagcggcg gtgccaaaaa cattaagaag
     1141 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     1201 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     1261 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     1321 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     1381 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     1441 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     1501 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     1561 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     1621 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     1681 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     1741 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     1801 cagatcatcc ccgccgagta ctgtttaagc tatgaaacgg aaatattgac agtagaatat
     1861 ggattattac cgattggtaa aattgtagaa aagcgcatcg aatgtactgt ttatagcgtt
     1921 gataataatg gaaatattta tacacaacct gtagcacaat ggcacgatcg cggagaacaa
     1981 gaggtgtttg agtattgttt ggaagatggt tcattgattc gggcaacaaa agaccataag
     2041 tttatgactg ttgatggtca aatgttgcca attgatgaaa tatttgaacg tgaattggat
     2101 ttgatgcggg ttgataattt gccgaacggc ggcaagatcg ccgtcaattc tgcttgcaag
     2161 aactggttca gtagcttaag ccactttgtg atccacctta acagccacgg cttccctccc
     2221 gaggtggagg agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg
     2281 gatagacacc ctgctgcttg cgccagcgcc aggatcaacg tctaa
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