BBa_K1966001 1 BBa_K1966001 lacPromoter-xylB 2016-10-10T11:00:00Z 2016-10-13T06:24:59Z Pseudomonas putida TOL pathway (upper operon) This part contains xylB from Pseudomonas putida under the control of a lac promoter. xylB is benzyl alcohol dehydrogenase (BADH) from Pseudomonas putida. BADH converts a -CH2OH group of benzyl alcohol and its derivatives into -CHOH. This is a basic part that is used in BBa_K1966002 with xylA and xylM (xylene monooxygenase, XO) to generate aromatic aldehydes from toluene derivatives. false false _2433_ 16818 16818 9 false Constructed from synthetic DNA; inserted into pSB1C3 backbone via biobrick assembly. false Brandon Wilbanks annotation2492835 1 RBS (part BBa_B0034) range2492835 1 56 67 annotation2492837 1 T1 terminator (part BBa_B0010) range2492837 1 1169 1248 annotation2492834 1 lac Promoter (part BBa_R0011) range2492834 1 1 55 annotation2492836 1 xylB range2492836 1 68 1168 BBa_K1966003 1 BBa_K1966003 lacPromoter-xylA-xylM-lacPromoter-xylB 2016-10-10T11:00:00Z 2016-10-13T06:24:30Z Pseudomonas putida TOL pathway (upper operon) This part is a composite built from BBa_K1966000 and BBa_K1966001. Part contains Pseudomonas putida xylA and xylM (xylene monooxygenase) with xylB (benzyl alcohol dehydrogenase). Used for conversion of methyl groups on toluene and its derivatives to aldehyde groups for generation of aromatic aldehydes. false false _2433_ 16818 16818 9 false Constructed from parts BBa_K1966000 and BBa_K1966001 via Biobrick assembly false Brandon Wilbanks component2492844 1 BBa_K1966000 component2492849 1 BBa_K1966001 annotation2492849 1 BBa_K1966001 range2492849 1 2331 3578 annotation2492844 1 BBa_K1966000 range2492844 1 1 2322 BBa_K1966000 1 BBa_K1966000 lacPromoter-xylA-xylM 2016-10-10T11:00:00Z 2016-10-29T08:42:33Z Pseudomonas putida (TOL pathway, upper operon) This part contains xylA and xylM from Pseudomonas putida under the control of a lac promoter. xylA and xylM are subunits of P. putida xylene monooxygenase (XO). XO converts a CH3 group of toluene and its derivatives into CH2OH. This is a basic part that is used in BBa_K1966002 with xylM (benzyl alcohol dehydrogenase, BADH) to generate aromatic aldehydes from toluene derivatives. false false _2433_ 16818 16818 9 false xylA and xlyM were built together from synthesized DNA via Gibson assembly false Brandon Wilbanks annotation2492831 1 T1 Terminator (part BBa_B0010) range2492831 1 2243 2322 annotation2492830 1 xylM range2492830 1 1133 2242 annotation2492829 1 RBS (part BBa_B0034) range2492829 1 1121 1132 annotation2492818 1 RBS (part BBa_B0034) range2492818 1 56 67 annotation2492819 1 xylA range2492819 1 68 1120 annotation2492817 1 lac promoter (part BBa_R0011) range2492817 1 1 55 BBa_K1966000_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaatgaacgagtttttcaaaaagatcagtgggcttttcgtacccccaccagagtcaactgtttcagtgcgcggtcaggggtttcaatttaaagtgccgcgtgggcagacgattttagaaagcgccttacatcagggcattgcgttcccacatgattgtaaggtggggtcgtgcggtacatgcaaatacaaattaatctctgggcgtgtcaacgaactgacatctagcgcgatgggcttatcaggagatttatatcaatcaggttaccgtctgggttgccaatgcatccctaaagaggacttagaaattgaactggataccgtactggggcaagcccttgtgcctattgagacttccgcactgatttccaaacaaaaacgtttggcacacgacatcgtagagatggaagtcgtgccggataagcaaattgcgttttaccctggccagtatgccgacgtggaatgcgctgaatgctctgcggtgcgttcgtattcgttttcagccccgccgcaaccagacgggagtttgtcattccatgtacgtcttgtgccaggtggcgtcttctcggggtggttatttgggggcgatcgcactggggctaccctgacactgcgcgcaccctatgggcaattcggtctgcacgaatcgaacgcaaccatggtctgtgtggcgggcggtacggggttggcgcctatcaaatgcgtattgcaatccatgacacaggcgcaacgtgaacgtgatgtcttactttttttcggagctcgccagcagcgtgatctttattgccttgacgaaatcgaagcgcttcaattagattggggtgggcgcttcgagttgattccggtcttatctgaggagtcgtcgaccagtagttggaagggtaaacgcggtatggtgactgaatatttcaaagaatacctgacggggcagccttatgaaggatacttgtgtggcccacccccaatggttgatgccgccgaaaccgagcttgtgcgtttaggcgttgctcgcgagttagtgtttgctgatcgtttttataatcgcccaccctgctagaaagaggagaaaatggacaccttacgctactacttaattcctgtcgtgaccgcctgtggattgattggattctattacgggggatactgggtctggcttggcgccgcgactttcccagccttaatggttttggacgtaatcttacccaaggatttcagtgcgcgtaaggtgtccccttttttcgcagacttaacgcagtatcttcaattaccactgatgattggtttgtatggccttttagtttttggagtagagaatggccgtattgagctgtctgagccgttgcaagtggcgggttgtatcctttcattggcgtggctgtcaggggtacctaccctgccggtgagtcacgagttgatgcaccgtcgccattggcttccacgtaagatggcacagttgttagcaatgttctatggggacccaaatcgtgacattgcgcacgttaatacccaccatttataccttgacacgcctctggattcggatacaccgtatcgcggtcaaacgatctactccttcgtaattagcgcaacagtaggtagcgtgaaggacgcgattaagatcgaagcagaaactcttcgtcgtaaaggccaatcgccctggaatctttcgaacaagacctaccaatacgttgccttacttctggcgttaccgggccttgtctcatatttgggtgggccggcgctggggctggtcacaatcgcatcgatgatcattgcgaaaggcattgtcgaagggtttaattacttccaacactacggtctggtgcgtgatcttgaccaaccgattttgttgcaccacgcatggaatcacatggggacaatcgtccgtccattaggatgtgaaatcactaaccatatcaaccaccatattgatggatacacgcgcttctacgaattacgcccagagaaggaggctccgcaaatgccatcgctgttcgtgtgtttcttgcttggattgattccgcccctttggttcgccttaatcgccaagcctaaacttcgcgattgggaccaacgctatgctactccgggcgaacgcgaattggcaatggctgcgaataaaaaagcaggatggccgttatggtgcgaaagtgagttggggcgcgtcgcgtctatctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1966003_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaatgaacgagtttttcaaaaagatcagtgggcttttcgtacccccaccagagtcaactgtttcagtgcgcggtcaggggtttcaatttaaagtgccgcgtgggcagacgattttagaaagcgccttacatcagggcattgcgttcccacatgattgtaaggtggggtcgtgcggtacatgcaaatacaaattaatctctgggcgtgtcaacgaactgacatctagcgcgatgggcttatcaggagatttatatcaatcaggttaccgtctgggttgccaatgcatccctaaagaggacttagaaattgaactggataccgtactggggcaagcccttgtgcctattgagacttccgcactgatttccaaacaaaaacgtttggcacacgacatcgtagagatggaagtcgtgccggataagcaaattgcgttttaccctggccagtatgccgacgtggaatgcgctgaatgctctgcggtgcgttcgtattcgttttcagccccgccgcaaccagacgggagtttgtcattccatgtacgtcttgtgccaggtggcgtcttctcggggtggttatttgggggcgatcgcactggggctaccctgacactgcgcgcaccctatgggcaattcggtctgcacgaatcgaacgcaaccatggtctgtgtggcgggcggtacggggttggcgcctatcaaatgcgtattgcaatccatgacacaggcgcaacgtgaacgtgatgtcttactttttttcggagctcgccagcagcgtgatctttattgccttgacgaaatcgaagcgcttcaattagattggggtgggcgcttcgagttgattccggtcttatctgaggagtcgtcgaccagtagttggaagggtaaacgcggtatggtgactgaatatttcaaagaatacctgacggggcagccttatgaaggatacttgtgtggcccacccccaatggttgatgccgccgaaaccgagcttgtgcgtttaggcgttgctcgcgagttagtgtttgctgatcgtttttataatcgcccaccctgctagaaagaggagaaaatggacaccttacgctactacttaattcctgtcgtgaccgcctgtggattgattggattctattacgggggatactgggtctggcttggcgccgcgactttcccagccttaatggttttggacgtaatcttacccaaggatttcagtgcgcgtaaggtgtccccttttttcgcagacttaacgcagtatcttcaattaccactgatgattggtttgtatggccttttagtttttggagtagagaatggccgtattgagctgtctgagccgttgcaagtggcgggttgtatcctttcattggcgtggctgtcaggggtacctaccctgccggtgagtcacgagttgatgcaccgtcgccattggcttccacgtaagatggcacagttgttagcaatgttctatggggacccaaatcgtgacattgcgcacgttaatacccaccatttataccttgacacgcctctggattcggatacaccgtatcgcggtcaaacgatctactccttcgtaattagcgcaacagtaggtagcgtgaaggacgcgattaagatcgaagcagaaactcttcgtcgtaaaggccaatcgccctggaatctttcgaacaagacctaccaatacgttgccttacttctggcgttaccgggccttgtctcatatttgggtgggccggcgctggggctggtcacaatcgcatcgatgatcattgcgaaaggcattgtcgaagggtttaattacttccaacactacggtctggtgcgtgatcttgaccaaccgattttgttgcaccacgcatggaatcacatggggacaatcgtccgtccattaggatgtgaaatcactaaccatatcaaccaccatattgatggatacacgcgcttctacgaattacgcccagagaaggaggctccgcaaatgccatcgctgttcgtgtgtttcttgcttggattgattccgcccctttggttcgccttaatcgccaagcctaaacttcgcgattgggaccaacgctatgctactccgggcgaacgcgaattggcaatggctgcgaataaaaaagcaggatggccgttatggtgcgaaagtgagttggggcgcgtcgcgtctatctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaatggaaatcaaagcggccatcgttcgtcagaaaaatggtccatttttgttagaacacgttgcccttaacgagccagccgaagatcaggtactggttcgtttagtcgcaactggtttatgtcatacagatcttgtatgtcgcgatcagcactatcctgtccctcttccaatggtattcgggcatgaaggggcaggggtggtggaacgtgtaggcagcgcggtaaagaaagtccagccgggggatcatgtggtcttaacattctatacgtgcggaagttgtgacgcatgccttagtggggaccccaccagctgcgcaaactcgttcggacctaatttcatgggacgtagcgttacgggcgaatgtactatccacgatcatcagggagccgaggtcggcgcctcattcttcgggcaaagcagctttgcgacatacgcgctttcttatgagcgtaatactgttaaggtgaccaaagatgtgccgctggagctgttggggcccttaggatgtgggattcaaacgggcgctggttctgtattgaatgcactgaatcccccggcgggatctgcgatcgcaatcttcggggctggggctgtgggcctgagtgcggttatggccgcggtggtggctggatgcactacaattattgctgtcgatgtcaaggaaaatcgccttgaattagcttcagaacttggagcaactcatattatcaatccggccgctaatgaccccatcgaagcgatcaaagaaatttttgccgacggtgtgccctacgttttggagacctctgggcttccggctgtattaacgcaagctatcctgtctagcgcaatcgggggagaaatcgggattgtgggggcgccgcctatgggcgctactgttcctgtagacatcaatttcttactgtttaatcgtaaattgcgcggaattgttgaagggcaatcaatttcagacatctttatcccccgcctggttgagttatatcgtcagggcaagttcccttttgataaactgattaaattttacccttttgacgaaatcaatcgcgcggccgaagattctgagaaaggagttacgttgaagcctgtcttgcgcatcggctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1966001_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaatggaaatcaaagcggccatcgttcgtcagaaaaatggtccatttttgttagaacacgttgcccttaacgagccagccgaagatcaggtactggttcgtttagtcgcaactggtttatgtcatacagatcttgtatgtcgcgatcagcactatcctgtccctcttccaatggtattcgggcatgaaggggcaggggtggtggaacgtgtaggcagcgcggtaaagaaagtccagccgggggatcatgtggtcttaacattctatacgtgcggaagttgtgacgcatgccttagtggggaccccaccagctgcgcaaactcgttcggacctaatttcatgggacgtagcgttacgggcgaatgtactatccacgatcatcagggagccgaggtcggcgcctcattcttcgggcaaagcagctttgcgacatacgcgctttcttatgagcgtaatactgttaaggtgaccaaagatgtgccgctggagctgttggggcccttaggatgtgggattcaaacgggcgctggttctgtattgaatgcactgaatcccccggcgggatctgcgatcgcaatcttcggggctggggctgtgggcctgagtgcggttatggccgcggtggtggctggatgcactacaattattgctgtcgatgtcaaggaaaatcgccttgaattagcttcagaacttggagcaactcatattatcaatccggccgctaatgaccccatcgaagcgatcaaagaaatttttgccgacggtgtgccctacgttttggagacctctgggcttccggctgtattaacgcaagctatcctgtctagcgcaatcgggggagaaatcgggattgtgggggcgccgcctatgggcgctactgttcctgtagacatcaatttcttactgtttaatcgtaaattgcgcggaattgttgaagggcaatcaatttcagacatctttatcccccgcctggttgagttatatcgtcagggcaagttcccttttgataaactgattaaattttacccttttgacgaaatcaatcgcgcggccgaagattctgagaaaggagttacgttgaagcctgtcttgcgcatcggctgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z