BBa_K1966004 1 BBa_K1966004 lacPromoter-xylR-PuPromoter-RFP 2016-10-10T11:00:00Z 2016-10-13T06:25:21Z Psuedomonas putida TOL pathway (upper operon) This part contains RFP and the Pu promoter under its activator for characterization of promoter function. xylR is required for activation of Pu. Part was not shown to function by UTK 2016 iGEM team (reasons unknown). false false _2433_ 16818 16818 9 false Constructed from synthetic DNA via Biobrick assembly false Brandon Wilbanks annotation2492883 1 T1 Terminator (part BBa_B0010) range2492883 1 2857 2936 annotation2492882 1 RFP range2492882 1 2179 2856 annotation2492880 1 Pu Promoter range2492880 1 1847 2166 annotation2492852 1 lacPromoter (part BBa_R0011) range2492852 1 1 53 annotation2492879 1 T1 Terminator (part BBa_B0010) range2492879 1 1767 1846 annotation2492874 1 RBS (part BBa_B0034) range2492874 1 54 65 annotation2492875 1 xylR range2492875 1 66 1766 annotation2492881 1 RBS (part BBa_B0034) range2492881 1 2167 2178 BBa_K1966004_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaaaagaggagaaaatgtcattaacatacaagccaaagatgcagcatgaagacatgcaggatctttcctcccaaatccgctttgtggctgctgagggcaagatttggctgggagagcaacgtatgttggtaatgcagttatcgactctggccagcttccgccgcgaaattatctctcttattggcgtggagcgtgcgaaggggttttttttacgtcttggctaccagtcggggctgatggacgcggaacttgcacgtaaattgcgcccggcaatgcgtgaggaggaggtcttcttagcaggacctcaattgtacgctttaaaggggatggtaaaggtccgcttactgactatggatatcgcgatccgtgatggacgcttcaatgttgaagcagagtggattgactcgtttgaggtggacatttgtcgtactgaactgggattgatgaacgagccagtttgctggactgtattaggctacgcgtcgggttacggctcagcttttatgggccgtcgtatcatcttccaggaaacatcgtgccgcggctgcggggatgataaatgccttattgttgggaagacggctgaagaatggggtgacgtgtcaagctttgaggcgtacttcaagagcgaccccattgtcgatgagcgttatgaactacagactcaggttgctaatctgcgtaatcgcttaaagcaatacgatggccagtattatggcattggtcacagtccagcctacaaacgcatctgcgagacaattgataaggcggcacgcggacgcgtgtcggttcttttgctgggtgaaaccggagtcggcaaagaggtgattgcacgttcagtacacttacgttccgaacgcgcagaacaaccgtttgtagcagtgaactgcgcggctatccctccagacctgattgagtctgagttatttggagttgacaagggagcgtacacgggagccgtcaatgctcgtgcgggtcgttttgaacgcgctaatggggggaccatttttttagatgaagtaattgaactgacaccccgcgctcaggctactttgttgcgcgttttgcaagagggggagctggaacgtgtaggtggagatcgcacacgcaaagttgatgtccgtctgattactgctacgaatgagaacctggaggaagccgtcaaaatgggtcgctttcgcgctgacctttttttccgcttaaacgtattccccgttcacattcccccgttacgcgagcgtgtggaagacatcccgctgttagtagagcacttcttgcgtcgtcaccataaagagtacgggaagaagacacttggcttaagtgatcgtgccatggaagcctgtttacactaccagtggcccgggaatatccgcgagttagaaaacgccttggagcgcggcgtgatcttgaccgaaagtaatgaatccattaacgtcgagtcgttatttcccggactggctaccgctaccgagggagatcgtcttagttccgaaggtcgcctggaagaggagtcaggtgactcgtggtttcgtcagatcatcgaccagggggtcagcttggaggatttagaggcaggacttatgcgcacagctatggaccgctgtgggcaaaacattagccaggcagcacgtttgttaggcctgacacgtccagccatggcataccgtctgaaaaaacttgatcccagcttgagcgtcaaagccatggggcgctagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctccccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgcaaagaggagaaaatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z