BBa_K1968003 1 BBa_K1968003 PglaA inducible promoter: glucoamylase gene promoter (PglaA) from Aspergillus niger 2016-10-10T11:00:00Z 2016-10-11T01:14:51Z This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). After the part was cloned, its sequence was confirmed by Sanger Sequencing. The Aspergillus a-glucoamylase (GlaA) enzyme is involved in starch utilization. The glaA promoter is induced by starch, maltose, and low concentrations of glucose but repressed by xylose (1). Basal transcription of glaA requires only 214 bp upstream of the start codon, while a high-level transcription expression requires the upstream region including -562 and -318 bp in the promoter region. The glaA promoters among different Aspergillus species are conserved. (1)Fowler, T., Berka, R. M., & Ward, M. (1990). Regulation of the glaA gene of Aspergillus niger. Curr Genet, 18(6), 537-545. true false _2435_ 30109 30109 9 false This part contains Phytobricks overhangs E and C, because this promoter includes the RBS and was the second promoter in our specific plasmid design. false Alejandra annotation2492767 1 xxxxx range2492767 1 1 5 BBa_K1968003_sequence 1 gatgcgtctctctcagcttgaactccaatcggggggagtacatggaatggccccagtggcaggaatcacgacagttgatgaatttcggagcgaacagcgccagtctccttacggatgatttccgcaacgggacatatgagttcatcctgcagaataccgcggctttccacatctgatgccattggcggaggggtccggaggtcagggacctagccttatgaacgtaatgatggaagtgtctggcctcggcaaaggatatatagggtcataataagtagtactagttatattaatggaagggtatataccacgcgttggaccttgggacctgcattatagcttcccgttaggtataattaccgttgttatagcagccaatcaagccaccacgctcgaccggggacggcgaatccccgggaattgaaataaattgcaattcaggtcaatgcggccagcgattggacacatctccaaggcacagggccattctgcagtgccggtggattcagtgcaatttcccccgggccggcccgacaccgcgataggctggttcttccacaccaccggagattcgttctgaagagctgaagtggcgagatgttctctgcaggaattcaagctagatgctaagctagatgctaagcgatattgcatggcaatatgtgttgatgcatatgcatgtgcttcttccttcagcttcccctcgtgcagatgaggtttggctataaattgaagtggttggtcggggttccgtgaggggctgaagtgcttcctcccttttagacgcaactgagagcctgagcttcatccccagcatcattacacctcagcaaatgcgagagagacgcagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z