BBa_K1968008
1
ErmE
ErmE promoter Phytobrick
2016-10-11T11:00:00Z
2016-10-19T03:05:34Z
This part was extracted through PCR from pCRISPR-Cas9 vector (GenBank accession number KR011749) (Tong et al., 2015) and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500 or pUPD2). The sequence of this part was confirmed by Sanger Sequencing.
A widely used constitutive promoter, ErmE (promoter region of erythromycin-resistance gene) was isolated from Streptomycin (Bibb et al., 1985). It works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM???s pairwise assembly standard.
References:
Bibb, M.J., Janssen, G.R. and Ward, J.M., 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene, 38, pp.215-226.
Tong, Y., Charusanti, P., Zhang, L., Weber, T. and Lee, S.Y., 2015. CRISPR-Cas9 based engineering of actinomycetal genomes. ACS synthetic biology, 4, pp.1020-1029.
false
false
_2435_
30015
30015
9
false
The part was integrated in reverse and we advise the user to use the reverse complement sequence for downstream applications.
false
Ibrahim Al-Masoud
annotation2494636
1
Phytobrick Adapter Site A
range2494636
1
1
4
annotation2494637
1
ErmE
range2494637
1
5
70
annotation2494638
1
Phytobrick Adapter Site B
range2494638
1
71
74
BBa_K1968008_sequence
1
agtaatggacgtccccttcctggacaccccggggcacgccgcgtttacttcaatgcgtgctcgtagctctctcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z