BBa_K1968009
1
BBa_K1968009
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
2016-10-11T11:00:00Z
2016-10-17T10:55:18Z
This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing
The Aspergillus a-glucoamylase (GlaA) enzyme is involved in starch utilization. The glaA promoter is induced by starch, maltose, and low concentrations of glucose but repressed by xylose (Fowler, Berka, & Ward, 1990). Basal transcription of glaA requires only 214 bp upstream of the start codon, while a high-level transcription expression requires the upstream region including -562 and -318 bp in the promoter region. The glaA promoters among different Aspergillus species are conserved.
false
false
_2435_
9146
30078
9
false
Phytobrick format compatible with MoClo (Overhangs E and C)
false
Alejandra Adriana Schiavon Osorio
annotation2499086
1
PglaA
range2499086
1
5
847
annotation2499085
1
Phytobrick Adapter Site E
range2499085
1
1
4
annotation2499087
1
Phytobrick Adapter Site C
range2499087
1
848
851
BBa_K1968009_sequence
1
gatgcgtctctctcagcttgaactccaatcggggggagtacatggaatggccccagtggcaggaatcacgacagttgatgaatttcggagcgaacagcgccagtctccttacggatgatttccgcaacgggacatatgagttcatcctgcagaataccgcggctttccacatctgatgccattggcggaggggtccggaggtcagggacctagccttatgaacgtaatgatggaagtgtctggcctcggcaaaggatatatagggtcataataagtagtactagttatattaatggaagggtatataccacgcgttggaccttgggacctgcattatagcttcccgttaggtataattaccgttgttatagcagccaatcaagccaccacgctcgaccggggacggcgaatccccgggaattgaaataaattgcaattcaggtcaatgcggccagcgattggacacatctccaaggcacagggccattctgcagtgccggtggattcagtgcaatttcccccgggccggcccgacaccgcgataggctggttcttccacaccaccggagattcgttctgaagagctgaagtggcgagatgttctctgcaggaattcaagctagatgctaagctagatgctaagcgatattgcatggcaatatgtgttgatgcatatgcatgtgcttcttccttcagcttcccctcgtgcagatgaggtttggctataaattgaagtggttggtcggggttccgtgaggggctgaagtgcttcctcccttttagacgcaactgagagcctgagcttcatccccagcatcattacacctcagcaaatgcgagagagacgcagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z