BBa_K1968013
1
BBa_K1968013
PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige
2016-10-11T11:00:00Z
2016-10-17T10:56:53Z
Genomic Sequence. The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection.
Glyceraldehyde-3-phosphate dehydrogenase (GPDA) is an enzyme that catalyzes the sixth step of glycolysis. The gene gpdA is constitutively expressed in A. nidulans (Punt et al., 1990). Moreover, the gpdA promoter is able to drive robust transcription of heterologous genes. Intracellularly expressed model heterologous proteins like the E. coli b-galactosidase (lacZ) and b-glucuronidase (uiaA) under the control of the gpdA promoter can account for up to 10???25% of soluble protein in A. niger (Punt, Zegers, Busscher, Pouwels, & van den Hondel, 1991). The ability to drive constitutive tran- scription makes the gpdA promoter particularly useful for expression, under varying cultures conditions, of selection markers and reporter genes, many of which are from heterologous sources.
false
false
_2435_
9146
30078
9
false
Phytrobrick format and MoClo compatible (Overhangs E and C)
false
Alejandra Adriana Schiavon Osorio
annotation2499081
1
Phytobrick Adapter Site E
range2499081
1
1
4
annotation2499083
1
PgdaA
range2499083
1
5
677
annotation2499082
1
Phytobrick Adapter Site C
range2499082
1
678
681
BBa_K1968013_sequence
1
gatgtctgctcaagcggggtagctgttagtcaagctgcgatgaagtgggaaagctcgaactgaaaggttcaaaggaataagggatgggaaggatggagtatggatgtagcaaagtacttacttaggggaaataaaggttcttggatgggaagatgaatatactgaagatgggaaaagaaagagaaaagaaaagagcagctggtggggagagcaggaaaatatggcaacaaatgttggactgacgcaacgaccttgtcaaccccgccgacacaccgggcggacagacggggcaaagctgcctaccagggactgagggacctcagcaggtcgagtgcagagcaccggatgggtcgactgccagcttgtgttcccggtctgcgccgctggccagctcctgagcggcctttccggtttcatacaccgggcaaagcaggagaggcacgatatttggacgccctacagatgccggatgggccaattagggagcttacgcgccgggtactcgctctacctacttcggagaaggtactatctcgtgaatcttttaccagatcggaagcaattggacttctgtacctaggttaatggcatgctatttcgccgacggctatacacccctggcttcacattctccttcgcttactgccggtgattcgatgaagctccatattctccgaagct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z