BBa_K1968020 1 BBa_K1968020 Synechocystis L03 Promoter with RBS* (TetR repressible) Phytobrick 2016-10-13T11:00:00Z 2016-10-14T07:41:57Z The part is based on a Promoter library by Huang et al. 2013, A Promoter library was created for Synechocystis sp. strain PCC 6803, by Huang et al, using the BBa_R0040 promoter as a template. The L03 promoter comes from this library and was selected because it showed a moderate repression and high induction rates in Synechocystis in the presence of the repressor protein TetR. At the same time, this part includes the Rybosome binding site "RBS*", RBS* which was found to be stronger than BBa_B0034 in Synechocystis (Heidorn et al. 2011) The fact that TetR does not naturally exist in the cyanobacteria adds second layer of control over the independent behaviour of the promoter. Meaning that TetR will only interact with the operator regions in the L03 promoter(Huang et al. 2013b). Huang, H.-H. et al., 2013. Wide-dynamic-range promoters engineered for cyanobacteria. Journal of Biological Engineering, 7(1), p.10. Heidorn T, Camsund D, Huang HH, Lindberg P, Oliveira P, Stensj?? K, Lindblad P, 2011. Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol 2011, 497:539???579. false false _2435_ 9146 9146 9 false This part was designed using the Phytobrick standard. false Ricardo Camilo Ch??vez Mart??nez annotation2505175 1 Phytobrick Adapter Site C range2505175 1 76 79 annotation2505173 1 Phytobrick Adapter Site A range2505173 1 1 4 annotation2505174 1 L03 Promoter range2505174 1 5 60 annotation2505176 1 RBS* range2505176 1 61 75 BBa_K1968020_sequence 1 ggagtccctatcagtgatagagattgacatccctatcagtgatagatataatggccactatagtggaggttactaaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z