BBa_K1969006 1 BBa_K1969006 S. cerevisiae GPA2 with 5 amino acids from human G(s) alpha-subunit 2016-10-07T11:00:00Z 2016-10-14T10:10:44Z It's a combination of yeast Gpa2 coding sequence (from genomic DNA of S. cerevisiae), and human G(s) alpha-subunit (from genomic DNA of human). This is DNA sequence coding for S. cerevisiae guanine nucleotide-binding protein subunit alpha (GPA2) with 5 amino acids at C terminus replaced by corresponding part of human G(s) alpha-subunit (alpha-S1). The strategy of making yeast???human chimeric G-protein, in which the carboxyl-terminal 5 amino acid residues of yeast G alpha subunit are replaced by the equivalent residues from human Gs proteins, has been shown to improve signal transmission in GPCR engineered yeast according to recent literature. false false _2436_ 29963 29963 9 Not in stock false The endogenous G proteins may not be able to couple with the mammalian GPCRs efficiently, so we do the modifications at C terminal residues to yield better results. false Yijie Lai annotation2506799 1 ATG range2506799 1 1 3 annotation2506798 1 Gpa2 (C terminus truncated) range2506798 1 1 1332 annotation2506801 1 TAA range2506801 1 1348 1350 annotation2506800 1 G(s) alpha (C terminus) range2506800 1 1333 1347 BBa_K1969006_sequence 1 atgggtctctgcgcatcttcagaaaagaacggcagcactcctgacacgcagaccgccagcgctggtagtgacaacgttggcaaagcgaaggtaccaccaaagcaggagccacagaagactgtgagaacagtcaacacagcaaatcaacaagaaaagcaacaacagaggcagcagcagccgtctccgcataatgttaaggaccgcaaggagcaaaacgggagcattaataacgcgatatctcccacggctacggcaaatacaagcggatcgcaacagatcaatatcgactctgccctgagagacaggtcgagtaacgttgcagcacaaccatcattgtcggacgcttcaagtggcagcaacgacaaagaactgaaagtgctactgctgggtgccggtgaaagtggtaagtccacggtattgcagcagttgaagattttacaccagaacgggtttagcgagcaggaaattaaagagtacatccccttgatctatcagaatctattggaaattggcaggaacctcatccaggcgagaacaaggtttaacgtcaacttggaaccggagtgtgaactgacgcaacaagacctgtcgagaaccatgtcctatgaaatgcccaataactacacgggccaattcccggaagatatcgcgggcgtaatatctacgttgtgggccttgccctcaacacaagatttagtcaatgggcctaacgcatcgaagttctatctaatggactcgactccttacttcatggaaaatttcaccaggatcacttcgcccaattacagacccacccagcaggacatattaagatcgagacagatgacgtcagggatttttgacaccgtcattgatatggggtcggatatcaagatgcatatttacgacgtgggtggacagcgttccgaaagaaaaaaatggatacactgcttcgacaatgtcactctggtcatattttgcgtttctctatcggagtacgaccagacgctgatggaggacaagaaccagaacaggtttcaggaatcgctggtgcttttcgataatattgtcaacagtagatggttcgcgcgcacgtctgtcgtactctttctgaataaaatcgacctttttgctgaaaaactaagcaaagtgcctatggaaaattacttcccagactacaccggcgggtcagacatcaacaaggctgctaagtacatactctggaggtttgttcagttaaacagggcgaatctaagcatatatcctcacgtgacacaggccacagacacgtcgaatataagattagtatttgccgccatcaaagaaacaattttggaaaatacattgaaagaccagtacgagctgctctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z