BBa_K1969019 1 BBa_K1969019 ADRB2 regulated by ADH1 promoter 2016-10-11T11:00:00Z 2016-10-12T06:56:16Z No. This is the scar left after inserting ADRB2 CDS into the sequence between GAL1 promoter and ADH1 terminator in original plasmid. false false _2436_ 29963 29963 9 Not in stock false No. false Yijie Lai component2495465 1 BBa_K1969015 component2495463 1 BBa_K1969003 component2495471 1 BBa_K1969009 component2495473 1 BBa_K1486025 component2495469 1 BBa_K1969001 annotation2495471 1 BBa_K1969009 range2495471 1 1979 2007 annotation2495473 1 BBa_K1486025 range2495473 1 2008 2195 annotation2495463 1 BBa_K1969003 range2495463 1 1 705 annotation2495465 1 BBa_K1969015 range2495465 1 706 736 annotation2495469 1 BBa_K1969001 range2495469 1 737 1978 BBa_K1969015 1 BBa_K1969015 The linker sequence between ADH1 promoter and ADRB2 2016-10-11T11:00:00Z 2016-10-12T06:52:10Z It is designed artificially. This DNA sequence is used to link the ADH1 promoter and ADRB2 together and introduce the restriction site for ClaI. false false _2436_ 29963 29963 9 false As we didn't have the enzyme required for biobrick assembly, we introduced the restriction site for ClaI. false Yijie Lai annotation2493924 1 Linker sequence between ADH1 promoter and ADRB2 range2493924 1 1 31 BBa_K1969003 1 BBa_K1969003 Full length S. cerevisiae ADH1 promoter 2016-10-04T11:00:00Z 2016-10-08T08:10:33Z a a false false _2436_ 29963 29963 9 false a false Yijie Lai annotation2486020 1 ADH1 promoter range2486020 1 1 705 BBa_K1969009 1 BBa_K1969009 The linker sequence between ADH1 terminator and different coding sequences 2016-10-08T11:00:00Z 2016-10-12T06:28:04Z It's artificially designed . This is the linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) to introduce the AvrII and AscI unique cut sites. false false _2436_ 29963 29963 9 false We need AvrII and AscI unique cut sites to assemble the parts together. false Yijie Lai annotation2489076 1 Linker between ADH1 terminator and Nano-lantern(cAMP-1.6) range2489076 1 1 29 BBa_K1969001 1 BBa_K1969001 Homo sapiens adrenoceptor beta 2 (ADRB2) 2016-10-04T11:00:00Z 2016-10-08T07:19:49Z Its from human genomic sequence. Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. It has seven membrane-spanning helices with an extracellular N-terminus and an intracellular C-terminus like all other members from GPCR family. In its host cell, once stimulated by catecholamine (epinephrine/norepinephrine), ADRB2 will undergo conformational change and interact with heterotrimeric G proteins to make alpha subunit (Gs) disassociated, then Gs will activate the adenylyl cyclase and further result in the soaring intracellular cAMP level and activation of enzymes downstream. It is reported to be functionally expressed in many mammalian and insect cells. false false _2436_ 29963 29963 9 false First, it has no obvious tag for researchers to detect it directly, so we further add a 6xhis tag at its C terminus. Second, as the N terminus sequence may decide the membrane localization in different host cells, we used the C terminus sequence of GPCR RI7 and STE2 which were both reported to be successfully located to yeast membrane to replace the corresponding sequence of ADRB2. false Yijie Lai annotation2487761 1 TAA range2487761 1 1240 1242 annotation2487760 1 ATG range2487760 1 1 3 annotation2486006 1 ADRB2 range2486006 1 1 1242 BBa_K1486025 1 BBa_K1486025 ADH1 Terminator 2014-09-25T11:00:00Z 2015-05-08T01:10:42Z To edit To edit false false _1866_ 0 21042 9 Not in stock false To edit false EPFL iGem team 2014 annotation2418776 1 Adh1 terminator range2418776 1 1 188 BBa_K1969015_sequence 1 atctcatatacaatgtctatcccaatcgata BBa_K1486025_sequence 1 gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969009_sequence 1 cctaggtgaggcgcgccacttctaaataa BBa_K1969003_sequence 1 atccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact BBa_K1969019_sequence 1 atccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaactatctcatatacaatgtctatcccaatcgataatggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgtaacctaggtgaggcgcgccacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969001_sequence 1 atggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z