LOCUS       BBa_K1969020            2221 bp    DNA     linear       26-Nov-2024
DEFINITION  ADRB2 with His-tag regulated by ADH1 promoter
ACCESSION   BBa_K1969020
VERSION     BBa_K1969020.1  
FEATURES             Location/Qualifiers
     promoter        1..705
                     /label=ADH1 promoter
     promoter        1..705
                     /label=BBa_K1969003
     misc_feature    706..744
                     /label=Linker sequence between ADH1 promoter and  ADRB2 
                     (with His-tag)
     misc_feature    706..744
                     /label=BBa_K1969016
     CDS             745..1983
                     /label=ADRB2
     misc_feature    1984..2004
                     /label=6 x His tag
     misc_feature    745..747
                     /label=ATG
     misc_feature    2002..2004
                     /label=TAA
     CDS             745..2004
                     /label=BBa_K1969002
     misc_feature    2005..2033
                     /label=Linker between ADH1 terminator and 
                     Nano-lantern(cAMP-1.6)
     misc_feature    2005..2033
                     /label=BBa_K1969009
     misc_feature    2034..2221
                     /label=Adh1 terminator
     terminator      2034..2221
                     /label=BBa_K1486025
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactatctc atatacaatg
      721 tctatcccaa tcgatattaa ttaaatgggg caacccggga acggcagcgc cttcttgctg
      781 gcacccaata gaagccatgc gccggaccac gacgtcacgc agcaaaggga cgaggtgtgg
      841 gtggtgggca tgggcatcgt catgtctctc atcgtcctgg ccatcgtgtt tggcaatgtg
      901 ctggtcatca cagccattgc caagttcgag cgtctacaga cggtcaccaa ctacttcatc
      961 acttcactgg cctgtgctga tctggtcatg ggcctggcag tggtgccctt tggggccgcc
     1021 catattctta tgaaaatgtg gacttttggc aacttctggt gcgagttttg gacttccatt
     1081 gatgtgctgt gcgtcacggc cagcattgag accctgtgcg tgatcgcagt ggatcgctac
     1141 tttgccatta cttcaccttt caagtaccag agcctgctga ccaagaataa ggcccgggtg
     1201 atcattctga tggtgtggat tgtgtcaggc cttacctcct tcttgcccat tcagatgcac
     1261 tggtaccggg ccacccacca ggaagccatc aactgctatg ccaatgagac ctgctgtgac
     1321 ttcttcacga accaagccta tgccattgcc tcttccatcg tgtccttcta cgttcccctg
     1381 gtgatcatgg tcttcgtcta ctccagggtc tttcaggagg ccaaaaggca gctccagaag
     1441 attgacaaat ctgagggccg cttccatgtc cagaacctta gccaggtgga gcaggatggg
     1501 cggacggggc atggactccg cagatcttcc aagttctgct tgaaggagca caaagccctc
     1561 aagacgttag gcatcatcat gggcactttc accctctgct ggctgccctt cttcatcgtt
     1621 aacattgtgc atgtgatcca ggataacctc atccgtaagg aagtttacat cctcctaaat
     1681 tggataggct atgtcaattc tggtttcaat ccccttatct actgccggag cccagatttc
     1741 aggattgcct tccaggagct tctgtgcctg cgcaggtctt ctttgaaggc ctatgggaat
     1801 ggctactcca gcaacggcaa cacaggggag cagagtggat atcacgtgga acaggagaaa
     1861 gaaaataaac tgctgtgtga agacctccca ggcacggaag actttgtggg ccatcaaggt
     1921 actgtgccta gcgataacat tgattcacaa gggaggaatt gtagtacaaa tgactcactg
     1981 ctgcaccacc accaccacca ctaacctagg tgaggcgcgc cacttctaaa taagcgaatt
     2041 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
     2101 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
     2161 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
     2221 g
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