BBa_K1969020 1 BBa_K1969020 ADRB2 with His-tag regulated by ADH1 promoter 2016-10-11T11:00:00Z 2016-10-12T07:05:44Z It is artificially designed. It is the linker sequence between ADH1 terminator and ADRB2 downstream GAL1 promoter. false false _2436_ 29963 29963 9 false It should meet the requirements of registered iGEM parts. false Yijie Lai component2495482 1 BBa_K1969002 component2495477 1 BBa_K1969016 component2495484 1 BBa_K1969009 component2495486 1 BBa_K1486025 component2495475 1 BBa_K1969003 annotation2495486 1 BBa_K1486025 range2495486 1 2034 2221 annotation2495482 1 BBa_K1969002 range2495482 1 745 2004 annotation2495475 1 BBa_K1969003 range2495475 1 1 705 annotation2495477 1 BBa_K1969016 range2495477 1 706 744 annotation2495484 1 BBa_K1969009 range2495484 1 2005 2033 BBa_K1486025 1 BBa_K1486025 ADH1 Terminator 2014-09-25T11:00:00Z 2015-05-08T01:10:42Z To edit To edit false false _1866_ 0 21042 9 Not in stock false To edit false EPFL iGem team 2014 annotation2418776 1 Adh1 terminator range2418776 1 1 188 BBa_K1969002 1 BBa_K1969002 Homo sapiens adrenoceptor beta 2 (ADRB2) with 6 x His tag 2016-10-04T11:00:00Z 2016-10-08T07:54:52Z The sequence for Homo sapiens adrenoceptor beta 2 is from human genomic sequence, the sequence for 6 x his tag was added artificially. Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. While the natural protein doesn't contain any mark for us to visualize or quantify its expression, the 6 x his tag is for immunofluorescence and western blotting. false false _2436_ 29963 29963 9 false It may require site-specific mutagenesis to become assembly compatible. false Yijie Lai annotation2486008 1 6 x His tag range2486008 1 1240 1260 annotation2487885 1 TAA range2487885 1 1258 1260 annotation2487884 1 ATG range2487884 1 1 3 annotation2486007 1 ADRB2 range2486007 1 1 1239 BBa_K1969016 1 BBa_K1969016 The linker sequence between ADH1 promoter and ADRB2 (with His-tag) 2016-10-11T11:00:00Z 2016-10-12T09:31:24Z It is designed artificially. This DNA sequence is used to link the ADH1 terminator and ADRB2 together and introduce the restriction sites for AvrII and AscI. false false _2436_ 29963 29963 9 false We wanted to introduce the restriction sites for AvrII and AscI to make it suitable for other elements. false Yijie Lai annotation2493967 1 Linker sequence between ADH1 promoter and ADRB2 (with His-tag) range2493967 1 1 39 BBa_K1969009 1 BBa_K1969009 The linker sequence between ADH1 terminator and different coding sequences 2016-10-08T11:00:00Z 2016-10-12T06:28:04Z It's artificially designed . This is the linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) to introduce the AvrII and AscI unique cut sites. false false _2436_ 29963 29963 9 false We need AvrII and AscI unique cut sites to assemble the parts together. false Yijie Lai annotation2489076 1 Linker between ADH1 terminator and Nano-lantern(cAMP-1.6) range2489076 1 1 29 BBa_K1969003 1 BBa_K1969003 Full length S. cerevisiae ADH1 promoter 2016-10-04T11:00:00Z 2016-10-08T08:10:33Z a a false false _2436_ 29963 29963 9 false a false Yijie Lai annotation2486020 1 ADH1 promoter range2486020 1 1 705 BBa_K1969020_sequence 1 atccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaactatctcatatacaatgtctatcccaatcgatattaattaaatggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgcaccaccaccaccaccactaacctaggtgaggcgcgccacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1486025_sequence 1 gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969002_sequence 1 atggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgcaccaccaccaccaccactaa BBa_K1969009_sequence 1 cctaggtgaggcgcgccacttctaaataa BBa_K1969003_sequence 1 atccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact BBa_K1969016_sequence 1 atctcatatacaatgtctatcccaatcgatattaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z