BBa_K1969021
1
BBa_K1969021
ADRB2 regulated by GAL1 promoter
2016-10-11T11:00:00Z
2016-10-12T07:13:03Z
Combination of corresponding basic parts.
The sequence of ADRB2 is cloned downstream the yeast GAL1 promoter and ended with an ADH1 terminator to generate a expression cassette with median expression level in yeast Saccharomyces cerevisiae.
false
false
_2436_
29963
29963
9
false
The GAL1 promoter is used for median expression of ADRB2 gene.
false
Yijie Lai
component2495510
1
BBa_K1486025
component2495500
1
BBa_K1969004
component2495502
1
BBa_K1969017
component2495506
1
BBa_K1969001
component2495508
1
BBa_K1969011
annotation2495502
1
BBa_K1969017
range2495502
1
443
491
annotation2495506
1
BBa_K1969001
range2495506
1
492
1733
annotation2495508
1
BBa_K1969011
range2495508
1
1734
1753
annotation2495500
1
BBa_K1969004
range2495500
1
1
442
annotation2495510
1
BBa_K1486025
range2495510
1
1754
1941
BBa_K1969004
1
BBa_K1969004
GAL1 promoter
2016-10-04T11:00:00Z
2016-10-08T08:45:50Z
Saccharomyces cerevisiae genomic sequence.
Transcription terminator for alcohol dehydrogenase 1.
false
false
_2436_
29963
29963
9
false
We chose to PCR amplify it out of Saccharomyces cerevisiae genomic DNA.
false
Yijie Lai
annotation2487974
1
GAL1 promoter
range2487974
1
1
442
BBa_K1969001
1
BBa_K1969001
Homo sapiens adrenoceptor beta 2 (ADRB2)
2016-10-04T11:00:00Z
2016-10-08T07:19:49Z
Its from human genomic sequence.
Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. It has seven membrane-spanning helices with an extracellular N-terminus and an intracellular C-terminus like all other members from GPCR family. In its host cell, once stimulated by catecholamine (epinephrine/norepinephrine), ADRB2 will undergo conformational change and interact with heterotrimeric G proteins to make alpha subunit (Gs) disassociated, then Gs will activate the adenylyl cyclase and further result in the soaring intracellular cAMP level and activation of enzymes downstream. It is reported to be functionally expressed in many mammalian and insect cells.
false
false
_2436_
29963
29963
9
false
First, it has no obvious tag for researchers to detect it directly, so we further add a 6xhis tag at its C terminus. Second, as the N terminus sequence may decide the membrane localization in different host cells, we used the C terminus sequence of GPCR RI7 and STE2 which were both reported to be successfully located to yeast membrane to replace the corresponding sequence of ADRB2.
false
Yijie Lai
annotation2486006
1
ADRB2
range2486006
1
1
1242
annotation2487761
1
TAA
range2487761
1
1240
1242
annotation2487760
1
ATG
range2487760
1
1
3
BBa_K1969017
1
BBa_K1969017
The linker sequence between GAL1 promoter and ADRB2
2016-10-11T11:00:00Z
2016-10-12T09:43:26Z
It is designed artificially.
This DNA sequence is used to link the ADH1 terminator and ADRB2 (with His-tag) together and introduce the restriction sites for AscI and AvrII.
false
false
_2436_
29963
29963
9
false
Introduction of the restriction sites for AscI and AvrII may make the construct more suitable for other elements.
false
Yijie Lai
annotation2493982
1
Linker sequence between GAL1 promoter and ADRB2
range2493982
1
1
49
BBa_K1969011
1
BBa_K1969011
Linker sequence between ADH1 terminator and two coding sequences downstream GAL1 promoter
2016-10-09T11:00:00Z
2016-10-12T06:33:57Z
It is designed artificially.
This is the linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) (downstream GAL1 promoter).
false
false
_2436_
29963
29963
9
false
It is designed for homologous recombination with the backbone plasmid used to insert in the HIS3 site in yeast.
false
Yijie Lai
annotation2495427
1
Linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) (downstream GAL1 promoter)
range2495427
1
1
20
BBa_K1486025
1
BBa_K1486025
ADH1 Terminator
2014-09-25T11:00:00Z
2015-05-08T01:10:42Z
To edit
To edit
false
false
_1866_
0
21042
9
Not in stock
false
To edit
false
EPFL iGem team 2014
annotation2418776
1
Adh1 terminator
range2418776
1
1
188
BBa_K1969011_sequence
1
ggcgcgccacttctaaataa
BBa_K1486025_sequence
1
gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg
BBa_K1969004_sequence
1
cggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggag
BBa_K1969021_sequence
1
cggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagatgtcgtacgctgcaggtcgtgggattcctgggttaattaaatcgatgtatggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgtaaggcgcgccacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg
BBa_K1969017_sequence
1
atgtcgtacgctgcaggtcgtgggattcctgggttaattaaatcgatgt
BBa_K1969001_sequence
1
atggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z