BBa_K1969021 1 BBa_K1969021 ADRB2 regulated by GAL1 promoter 2016-10-11T11:00:00Z 2016-10-12T07:13:03Z Combination of corresponding basic parts. The sequence of ADRB2 is cloned downstream the yeast GAL1 promoter and ended with an ADH1 terminator to generate a expression cassette with median expression level in yeast Saccharomyces cerevisiae. false false _2436_ 29963 29963 9 false The GAL1 promoter is used for median expression of ADRB2 gene. false Yijie Lai component2495510 1 BBa_K1486025 component2495500 1 BBa_K1969004 component2495502 1 BBa_K1969017 component2495506 1 BBa_K1969001 component2495508 1 BBa_K1969011 annotation2495502 1 BBa_K1969017 range2495502 1 443 491 annotation2495506 1 BBa_K1969001 range2495506 1 492 1733 annotation2495508 1 BBa_K1969011 range2495508 1 1734 1753 annotation2495500 1 BBa_K1969004 range2495500 1 1 442 annotation2495510 1 BBa_K1486025 range2495510 1 1754 1941 BBa_K1969004 1 BBa_K1969004 GAL1 promoter 2016-10-04T11:00:00Z 2016-10-08T08:45:50Z Saccharomyces cerevisiae genomic sequence. Transcription terminator for alcohol dehydrogenase 1. false false _2436_ 29963 29963 9 false We chose to PCR amplify it out of Saccharomyces cerevisiae genomic DNA. false Yijie Lai annotation2487974 1 GAL1 promoter range2487974 1 1 442 BBa_K1969001 1 BBa_K1969001 Homo sapiens adrenoceptor beta 2 (ADRB2) 2016-10-04T11:00:00Z 2016-10-08T07:19:49Z Its from human genomic sequence. Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. It has seven membrane-spanning helices with an extracellular N-terminus and an intracellular C-terminus like all other members from GPCR family. In its host cell, once stimulated by catecholamine (epinephrine/norepinephrine), ADRB2 will undergo conformational change and interact with heterotrimeric G proteins to make alpha subunit (Gs) disassociated, then Gs will activate the adenylyl cyclase and further result in the soaring intracellular cAMP level and activation of enzymes downstream. It is reported to be functionally expressed in many mammalian and insect cells. false false _2436_ 29963 29963 9 false First, it has no obvious tag for researchers to detect it directly, so we further add a 6xhis tag at its C terminus. Second, as the N terminus sequence may decide the membrane localization in different host cells, we used the C terminus sequence of GPCR RI7 and STE2 which were both reported to be successfully located to yeast membrane to replace the corresponding sequence of ADRB2. false Yijie Lai annotation2486006 1 ADRB2 range2486006 1 1 1242 annotation2487761 1 TAA range2487761 1 1240 1242 annotation2487760 1 ATG range2487760 1 1 3 BBa_K1969017 1 BBa_K1969017 The linker sequence between GAL1 promoter and ADRB2 2016-10-11T11:00:00Z 2016-10-12T09:43:26Z It is designed artificially. This DNA sequence is used to link the ADH1 terminator and ADRB2 (with His-tag) together and introduce the restriction sites for AscI and AvrII. false false _2436_ 29963 29963 9 false Introduction of the restriction sites for AscI and AvrII may make the construct more suitable for other elements. false Yijie Lai annotation2493982 1 Linker sequence between GAL1 promoter and ADRB2 range2493982 1 1 49 BBa_K1969011 1 BBa_K1969011 Linker sequence between ADH1 terminator and two coding sequences downstream GAL1 promoter 2016-10-09T11:00:00Z 2016-10-12T06:33:57Z It is designed artificially. This is the linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) (downstream GAL1 promoter). false false _2436_ 29963 29963 9 false It is designed for homologous recombination with the backbone plasmid used to insert in the HIS3 site in yeast. false Yijie Lai annotation2495427 1 Linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) (downstream GAL1 promoter) range2495427 1 1 20 BBa_K1486025 1 BBa_K1486025 ADH1 Terminator 2014-09-25T11:00:00Z 2015-05-08T01:10:42Z To edit To edit false false _1866_ 0 21042 9 Not in stock false To edit false EPFL iGem team 2014 annotation2418776 1 Adh1 terminator range2418776 1 1 188 BBa_K1969011_sequence 1 ggcgcgccacttctaaataa BBa_K1486025_sequence 1 gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969004_sequence 1 cggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggag BBa_K1969021_sequence 1 cggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagatgtcgtacgctgcaggtcgtgggattcctgggttaattaaatcgatgtatggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgtaaggcgcgccacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969017_sequence 1 atgtcgtacgctgcaggtcgtgggattcctgggttaattaaatcgatgt BBa_K1969001_sequence 1 atggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z