LOCUS       BBa_K1969022            1960 bp    DNA     linear       22-Nov-2024
DEFINITION  ADRB2 with His-tag regulated by GAL1 promoter
ACCESSION   BBa_K1969022
VERSION     BBa_K1969022.1  
FEATURES             Location/Qualifiers
     promoter        1..442
                     /label=GAL1 promoter
     promoter        1..442
                     /label=BBa_K1969004
     misc_feature    443..483
                     /label=Linker sequence between GAL1 promoter and  ADRB2 
                     (with His-tag)
     misc_feature    443..483
                     /label=BBa_K1969018
     CDS             484..1722
                     /label=ADRB2
     misc_feature    1723..1743
                     /label=6 x His tag
     misc_feature    484..486
                     /label=ATG
     misc_feature    1741..1743
                     /label=TAA
     CDS             484..1743
                     /label=BBa_K1969002
     misc_feature    1744..1772
                     /label=Linker between ADH1 terminator and 
                     Nano-lantern(cAMP-1.6)
     misc_feature    1744..1772
                     /label=BBa_K1969009
     misc_feature    1773..1960
                     /label=Adh1 terminator
     terminator      1773..1960
                     /label=BBa_K1486025
ORIGIN
        1 cggattagaa gccgccgagc gggcgacagc cctccgacgg aagactctcc tccgtgcgtc
       61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      181 tggccccaca aaccttcaaa ttaacgaatc aaattaacaa ccataggatg ataatgcgat
      241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      301 aacagatata taaatggaaa agctgcataa ccactttaac taatactttc aacattttca
      361 gtttgtatta cttcttattc aaatgtcata aaagtatcaa caaaaaattg ttaatatacc
      421 tctatacttt aacgtcaagg agatgtcgta cgctgcaggt cgtgggattc ctgggttaat
      481 taaatggggc aacccgggaa cggcagcgcc ttcttgctgg cacccaatag aagccatgcg
      541 ccggaccacg acgtcacgca gcaaagggac gaggtgtggg tggtgggcat gggcatcgtc
      601 atgtctctca tcgtcctggc catcgtgttt ggcaatgtgc tggtcatcac agccattgcc
      661 aagttcgagc gtctacagac ggtcaccaac tacttcatca cttcactggc ctgtgctgat
      721 ctggtcatgg gcctggcagt ggtgcccttt ggggccgccc atattcttat gaaaatgtgg
      781 acttttggca acttctggtg cgagttttgg acttccattg atgtgctgtg cgtcacggcc
      841 agcattgaga ccctgtgcgt gatcgcagtg gatcgctact ttgccattac ttcacctttc
      901 aagtaccaga gcctgctgac caagaataag gcccgggtga tcattctgat ggtgtggatt
      961 gtgtcaggcc ttacctcctt cttgcccatt cagatgcact ggtaccgggc cacccaccag
     1021 gaagccatca actgctatgc caatgagacc tgctgtgact tcttcacgaa ccaagcctat
     1081 gccattgcct cttccatcgt gtccttctac gttcccctgg tgatcatggt cttcgtctac
     1141 tccagggtct ttcaggaggc caaaaggcag ctccagaaga ttgacaaatc tgagggccgc
     1201 ttccatgtcc agaaccttag ccaggtggag caggatgggc ggacggggca tggactccgc
     1261 agatcttcca agttctgctt gaaggagcac aaagccctca agacgttagg catcatcatg
     1321 ggcactttca ccctctgctg gctgcccttc ttcatcgtta acattgtgca tgtgatccag
     1381 gataacctca tccgtaagga agtttacatc ctcctaaatt ggataggcta tgtcaattct
     1441 ggtttcaatc cccttatcta ctgccggagc ccagatttca ggattgcctt ccaggagctt
     1501 ctgtgcctgc gcaggtcttc tttgaaggcc tatgggaatg gctactccag caacggcaac
     1561 acaggggagc agagtggata tcacgtggaa caggagaaag aaaataaact gctgtgtgaa
     1621 gacctcccag gcacggaaga ctttgtgggc catcaaggta ctgtgcctag cgataacatt
     1681 gattcacaag ggaggaattg tagtacaaat gactcactgc tgcaccacca ccaccaccac
     1741 taacctaggt gaggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     1801 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     1861 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     1921 tatagtatga ggtcgctctt attgaccaca cctctaccgg
//