LOCUS BBa_K1969022 1960 bp DNA linear 23-Dec-2024 DEFINITION ADRB2 with His-tag regulated by GAL1 promoter ACCESSION BBa_K1969022 VERSION BBa_K1969022.1 FEATURES Location/Qualifiers promoter 1..442 /label=GAL1 promoter promoter 1..442 /label=BBa_K1969004 misc_feature 443..483 /label=Linker sequence between GAL1 promoter and ADRB2 (with His-tag) misc_feature 443..483 /label=BBa_K1969018 CDS 484..1722 /label=ADRB2 misc_feature 1723..1743 /label=6 x His tag misc_feature 484..486 /label=ATG misc_feature 1741..1743 /label=TAA CDS 484..1743 /label=BBa_K1969002 misc_feature 1744..1772 /label=Linker between ADH1 terminator and Nano-lantern(cAMP-1.6) misc_feature 1744..1772 /label=BBa_K1969009 misc_feature 1773..1960 /label=Adh1 terminator terminator 1773..1960 /label=BBa_K1486025 ORIGIN 1 cggattagaa gccgccgagc gggcgacagc cctccgacgg aagactctcc tccgtgcgtc 61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa 121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc 181 tggccccaca aaccttcaaa ttaacgaatc aaattaacaa ccataggatg ataatgcgat 241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt 301 aacagatata taaatggaaa agctgcataa ccactttaac taatactttc aacattttca 361 gtttgtatta cttcttattc aaatgtcata aaagtatcaa caaaaaattg ttaatatacc 421 tctatacttt aacgtcaagg agatgtcgta cgctgcaggt cgtgggattc ctgggttaat 481 taaatggggc aacccgggaa cggcagcgcc ttcttgctgg cacccaatag aagccatgcg 541 ccggaccacg acgtcacgca gcaaagggac gaggtgtggg tggtgggcat gggcatcgtc 601 atgtctctca tcgtcctggc catcgtgttt ggcaatgtgc tggtcatcac agccattgcc 661 aagttcgagc gtctacagac ggtcaccaac tacttcatca cttcactggc ctgtgctgat 721 ctggtcatgg gcctggcagt ggtgcccttt ggggccgccc atattcttat gaaaatgtgg 781 acttttggca acttctggtg cgagttttgg acttccattg atgtgctgtg cgtcacggcc 841 agcattgaga ccctgtgcgt gatcgcagtg gatcgctact ttgccattac ttcacctttc 901 aagtaccaga gcctgctgac caagaataag gcccgggtga tcattctgat ggtgtggatt 961 gtgtcaggcc ttacctcctt cttgcccatt cagatgcact ggtaccgggc cacccaccag 1021 gaagccatca actgctatgc caatgagacc tgctgtgact tcttcacgaa ccaagcctat 1081 gccattgcct cttccatcgt gtccttctac gttcccctgg tgatcatggt cttcgtctac 1141 tccagggtct ttcaggaggc caaaaggcag ctccagaaga ttgacaaatc tgagggccgc 1201 ttccatgtcc agaaccttag ccaggtggag caggatgggc ggacggggca tggactccgc 1261 agatcttcca agttctgctt gaaggagcac aaagccctca agacgttagg catcatcatg 1321 ggcactttca ccctctgctg gctgcccttc ttcatcgtta acattgtgca tgtgatccag 1381 gataacctca tccgtaagga agtttacatc ctcctaaatt ggataggcta tgtcaattct 1441 ggtttcaatc cccttatcta ctgccggagc ccagatttca ggattgcctt ccaggagctt 1501 ctgtgcctgc gcaggtcttc tttgaaggcc tatgggaatg gctactccag caacggcaac 1561 acaggggagc agagtggata tcacgtggaa caggagaaag aaaataaact gctgtgtgaa 1621 gacctcccag gcacggaaga ctttgtgggc catcaaggta ctgtgcctag cgataacatt 1681 gattcacaag ggaggaattg tagtacaaat gactcactgc tgcaccacca ccaccaccac 1741 taacctaggt gaggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta 1801 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt 1861 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg 1921 tatagtatga ggtcgctctt attgaccaca cctctaccgg //