BBa_K1969022 1 BBa_K1969022 ADRB2 with His-tag regulated by GAL1 promoter 2016-10-11T11:00:00Z 2016-10-12T07:19:05Z Combination of corresponding basic parts. The sequence of ADRB2 with His-tag at C terminus is cloned downstream the yeast GAL1 promoter and ended with an ADH1 terminator to generate a median expression cassette in yeast Saccharomyces cerevisiae. false false _2436_ 29963 29963 9 false The His-tag is used to monitor the expression and membrane localization of ADRB2 gene. false Yijie Lai component2495594 1 BBa_K1969004 component2495605 1 BBa_K1486025 component2495603 1 BBa_K1969009 component2495596 1 BBa_K1969018 component2495601 1 BBa_K1969002 annotation2495594 1 BBa_K1969004 range2495594 1 1 442 annotation2495603 1 BBa_K1969009 range2495603 1 1744 1772 annotation2495605 1 BBa_K1486025 range2495605 1 1773 1960 annotation2495596 1 BBa_K1969018 range2495596 1 443 483 annotation2495601 1 BBa_K1969002 range2495601 1 484 1743 BBa_K1969018 1 BBa_K1969018 The linker sequence between GAL1 promoter and ADRB2 (with His-tag) 2016-10-11T11:00:00Z 2016-10-12T09:51:54Z It is designed artificially. This DNA sequence is used to link the GAL1 promoter and ADRB2 (with His-tag) together and introduce the restriction site for PacI. false false _2436_ 29963 29963 9 false Introduction of the restriction sites for PacI may make the construct more suitable for other elements. false Yijie Lai annotation2494019 1 Linker sequence between GAL1 promoter and ADRB2 (with His-tag) range2494019 1 1 41 BBa_K1969009 1 BBa_K1969009 The linker sequence between ADH1 terminator and different coding sequences 2016-10-08T11:00:00Z 2016-10-12T06:28:04Z It's artificially designed . This is the linker sequence between ADH1 terminator and Nano-lantern(cAMP-1.6) to introduce the AvrII and AscI unique cut sites. false false _2436_ 29963 29963 9 false We need AvrII and AscI unique cut sites to assemble the parts together. false Yijie Lai annotation2489076 1 Linker between ADH1 terminator and Nano-lantern(cAMP-1.6) range2489076 1 1 29 BBa_K1486025 1 BBa_K1486025 ADH1 Terminator 2014-09-25T11:00:00Z 2015-05-08T01:10:42Z To edit To edit false false _1866_ 0 21042 9 Not in stock false To edit false EPFL iGem team 2014 annotation2418776 1 Adh1 terminator range2418776 1 1 188 BBa_K1969004 1 BBa_K1969004 GAL1 promoter 2016-10-04T11:00:00Z 2016-10-08T08:45:50Z Saccharomyces cerevisiae genomic sequence. Transcription terminator for alcohol dehydrogenase 1. false false _2436_ 29963 29963 9 false We chose to PCR amplify it out of Saccharomyces cerevisiae genomic DNA. false Yijie Lai annotation2487974 1 GAL1 promoter range2487974 1 1 442 BBa_K1969002 1 BBa_K1969002 Homo sapiens adrenoceptor beta 2 (ADRB2) with 6 x His tag 2016-10-04T11:00:00Z 2016-10-08T07:54:52Z The sequence for Homo sapiens adrenoceptor beta 2 is from human genomic sequence, the sequence for 6 x his tag was added artificially. Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. While the natural protein doesn't contain any mark for us to visualize or quantify its expression, the 6 x his tag is for immunofluorescence and western blotting. false false _2436_ 29963 29963 9 false It may require site-specific mutagenesis to become assembly compatible. false Yijie Lai annotation2486007 1 ADRB2 range2486007 1 1 1239 annotation2487885 1 TAA range2487885 1 1258 1260 annotation2486008 1 6 x His tag range2486008 1 1240 1260 annotation2487884 1 ATG range2487884 1 1 3 BBa_K1486025_sequence 1 gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969004_sequence 1 cggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggag BBa_K1969022_sequence 1 cggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagatgtcgtacgctgcaggtcgtgggattcctgggttaattaaatggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgcaccaccaccaccaccactaacctaggtgaggcgcgccacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg BBa_K1969002_sequence 1 atggggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggccatcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctacagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttctggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccttacctccttcttgcccattcagatgcactggtaccgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggagcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagcgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgcaccaccaccaccaccactaa BBa_K1969018_sequence 1 atgtcgtacgctgcaggtcgtgggattcctgggttaattaa BBa_K1969009_sequence 1 cctaggtgaggcgcgccacttctaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z