LOCUS       BBa_K1969024            2311 bp    DNA     linear       05-May-2025
DEFINITION  C terminus engineered Gpa2 protein regulated by ADH1 promoter
ACCESSION   BBa_K1969024
VERSION     BBa_K1969024.1  
FEATURES             Location/Qualifiers
     promoter        1..705
                     /label=ADH1 promoter
     promoter        1..705
                     /label=BBa_K1969003
     misc_feature    706..744
                     /label=Linker sequence between ADH1 promoter and  ADRB2 
                     (with His-tag)
     misc_feature    706..744
                     /label=BBa_K1969016
     CDS             745..2076
                     /label=Gpa2 (C terminus truncated)
     misc_feature    745..747
                     /label=ATG
     CDS             2077..2091
                     /label=G(s) alpha (C terminus)
     misc_feature    2092..2094
                     /label=TAA
     CDS             745..2094
                     /label=BBa_K1969006
     misc_feature    2095..2123
                     /label=Linker between ADH1 terminator and 
                     Nano-lantern(cAMP-1.6)
     misc_feature    2095..2123
                     /label=BBa_K1969009
     misc_feature    2124..2311
                     /label=Adh1 terminator
     terminator      2124..2311
                     /label=BBa_K1486025
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactatctc atatacaatg
      721 tctatcccaa tcgatattaa ttaaatgggt ctctgcgcat cttcagaaaa gaacggcagc
      781 actcctgaca cgcagaccgc cagcgctggt agtgacaacg ttggcaaagc gaaggtacca
      841 ccaaagcagg agccacagaa gactgtgaga acagtcaaca cagcaaatca acaagaaaag
      901 caacaacaga ggcagcagca gccgtctccg cataatgtta aggaccgcaa ggagcaaaac
      961 gggagcatta ataacgcgat atctcccacg gctacggcaa atacaagcgg atcgcaacag
     1021 atcaatatcg actctgccct gagagacagg tcgagtaacg ttgcagcaca accatcattg
     1081 tcggacgctt caagtggcag caacgacaaa gaactgaaag tgctactgct gggtgccggt
     1141 gaaagtggta agtccacggt attgcagcag ttgaagattt tacaccagaa cgggtttagc
     1201 gagcaggaaa ttaaagagta catccccttg atctatcaga atctattgga aattggcagg
     1261 aacctcatcc aggcgagaac aaggtttaac gtcaacttgg aaccggagtg tgaactgacg
     1321 caacaagacc tgtcgagaac catgtcctat gaaatgccca ataactacac gggccaattc
     1381 ccggaagata tcgcgggcgt aatatctacg ttgtgggcct tgccctcaac acaagattta
     1441 gtcaatgggc ctaacgcatc gaagttctat ctaatggact cgactcctta cttcatggaa
     1501 aatttcacca ggatcacttc gcccaattac agacccaccc agcaggacat attaagatcg
     1561 agacagatga cgtcagggat ttttgacacc gtcattgata tggggtcgga tatcaagatg
     1621 catatttacg acgtgggtgg acagcgttcc gaaagaaaaa aatggataca ctgcttcgac
     1681 aatgtcactc tggtcatatt ttgcgtttct ctatcggagt acgaccagac gctgatggag
     1741 gacaagaacc agaacaggtt tcaggaatcg ctggtgcttt tcgataatat tgtcaacagt
     1801 agatggttcg cgcgcacgtc tgtcgtactc tttctgaata aaatcgacct ttttgctgaa
     1861 aaactaagca aagtgcctat ggaaaattac ttcccagact acaccggcgg gtcagacatc
     1921 aacaaggctg ctaagtacat actctggagg tttgttcagt taaacagggc gaatctaagc
     1981 atatatcctc acgtgacaca ggccacagac acgtcgaata taagattagt atttgccgcc
     2041 atcaaagaaa caattttgga aaatacattg aaagaccagt acgagctgct ctgacctagg
     2101 tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat
     2161 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     2221 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg
     2281 aggtcgctct tattgaccac acctctaccg g
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