LOCUS       BBa_K1970000            2610 bp    DNA     linear       23-Dec-2024
DEFINITION  Deoxynucleotide ribonuclease expressing plasmid which cuts off 
            self inhibitory RBS tails
ACCESSION   BBa_K1970000
VERSION     BBa_K1970000.1  
FEATURES             Location/Qualifiers
     promoter        1..201
                     /label=lacI promoter
     CDS             413..1375
                     /label=reverse transcriptase
     misc_feature    283..393
                     /label=msDNA
     misc_feature    213..294
                     /label=misc RNA
     misc_feature    318..362
                     /label=DNAzyme
     promoter        1385..1438
                     /label=tetR promoter
     misc_feature    1447..1528
                     /label=misc RNA
     misc_feature    1518..1628
                     /label=msDNA
     misc_feature    1552..1596
                     /label=DNAzyme
     CDS             1648..2610
                     /label=reverse transcriptase
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagctag agatgcgcac ccttagcgag aggtttatca
      241 ttaaggtcaa cctctggatg ttgtttcggc atcctgcatt gaatctgagt tactgtctgt
      301 tttcctgaat tgtcgacggg gccgcgggag gctcgttgta gctagcctta tatatgggtt
      361 tgggtaccaa ttcaggaaac ccgttttttc tgacgtaagg gtgcgcaact ttcatgaaat
      421 ccgctgaata tttgaacact tttagattga gaaatctcgg cctacctgtc atgaacaatt
      481 tgcatgacat gtctaaggcg actcgcatat ctgttgaaac acttcggttg ttaatctata
      541 cagctgattt tcgctatagg atctacactg tagaaaagaa aggcccagag aagagaatga
      601 gaaccattta ccaaccttct cgagaactta aagccttaca aggatgggtt ctacgtaaca
      661 ttttagataa actgtcgtca tctccttttt ctattggatt tgaaaagcac caatctattt
      721 tgaataatgc taccccgcat attggggcaa actttatact gaatattgat ttggaggatt
      781 ttttcccaag tttaactgct aacaaagttt ttggagtgtt ccattctctt ggttataatc
      841 gactaatatc ttcagttttg acaaaaatat gttgttataa aaatctgcta ccacaaggtg
      901 ctccatcatc acctaaatta gctaatctaa tatgttctaa acttgattat cgtattcagg
      961 gttatgcagg tagtcggggc ttgatatata cgagatatgc cgatgacctc accttatctg
     1021 cacagtctat gaaaaaggtt gttaaagcac gtgatttttt attttctata atcccaagtg
     1081 aaggattggt tattaactca aaaaaaactt gtattagtgg gcctcgtagt cagaggaaag
     1141 ttacaggttt agttatttca caagagaaag ttgggatagg tagagaaaaa tataaagaaa
     1201 ttagagcaaa gatacatcat atattttgcg gtaagtcttc tgagatagaa cacgttaggg
     1261 gatggttgtc atttatttta agtgtggatt caaaaagcca taggagatta ataacttata
     1321 ttagcaaatt agaaaaaaaa tatggaaaga accctttaaa taaagcgaag acctaatact
     1381 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
     1441 ctagagatgc gcacccttag cgagaggttt atcattaagg tcaacctctg gatgttgttt
     1501 cggcatcctg cattgaatct gagttactgt ctgttttcct gaattgtcga ccaaaccgcg
     1561 ggaggctcgt tgtagctagc cttatatatg ggccccggta ccaattcagg aaacccgttt
     1621 tttctgacgt aagggtgcgc aactttcatg aaatccgctg aatatttgaa cacttttaga
     1681 ttgagaaatc tcggcctacc tgtcatgaac aatttgcatg acatgtctaa ggcgactcgc
     1741 atatctgttg aaacacttcg gttgttaatc tatacagctg attttcgcta taggatctac
     1801 actgtagaaa agaaaggccc agagaagaga atgagaacca tttaccaacc ttctcgagaa
     1861 cttaaagcct tacaaggatg ggttctacgt aacattttag ataaactgtc gtcatctcct
     1921 ttttctattg gatttgaaaa gcaccaatct attttgaata atgctacccc gcatattggg
     1981 gcaaacttta tactgaatat tgatttggag gattttttcc caagtttaac tgctaacaaa
     2041 gtttttggag tgttccattc tcttggttat aatcgactaa tatcttcagt tttgacaaaa
     2101 atatgttgtt ataaaaatct gctaccacaa ggtgctccat catcacctaa attagctaat
     2161 ctaatatgtt ctaaacttga ttatcgtatt cagggttatg caggtagtcg gggcttgata
     2221 tatacgagat atgccgatga cctcacctta tctgcacagt ctatgaaaaa ggttgttaaa
     2281 gcacgtgatt ttttattttc tataatccca agtgaaggat tggttattaa ctcaaaaaaa
     2341 acttgtatta gtgggcctcg tagtcagagg aaagttacag gtttagttat ttcacaagag
     2401 aaagttggga taggtagaga aaaatataaa gaaattagag caaagataca tcatatattt
     2461 tgcggtaagt cttctgagat agaacacgtt aggggatggt tgtcatttat tttaagtgtg
     2521 gattcaaaaa gccataggag attaataact tatattagca aattagaaaa aaaatatgga
     2581 aagaaccctt taaataaagc gaagacctaa
//