BBa_K1970000 1 BBa_K1970000 Deoxynucleotide ribonuclease expressing plasmid which cuts off self inhibitory RBS tails 2016-10-20T11:00:00Z 2016-10-27T07:18:27Z De novo DNA This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes. false false _2437_ 30065 20673 9 false None in particular. false Eivind B??e Drejer annotation2527128 1 DNAzyme range2527128 1 318 362 annotation2527131 1 msDNA range2527131 1 1518 1628 annotation2527133 1 reverse transcriptase range2527133 1 1648 2610 annotation2527130 1 misc RNA range2527130 1 1447 1528 annotation2527039 1 lacI promoter range2527039 1 1 201 annotation2527123 1 misc RNA range2527123 1 213 294 annotation2527132 1 DNAzyme range2527132 1 1552 1596 annotation2527056 1 reverse transcriptase range2527056 1 413 1375 annotation2527129 1 tetR promoter range2527129 1 1385 1438 annotation2527061 1 msDNA range2527061 1 283 393 BBa_K1970000_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagctagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgacggggccgcgggaggctcgttgtagctagccttatatatgggtttgggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgaccaaaccgcgggaggctcgttgtagctagccttatatatgggccccggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z