BBa_K1970001 1 BBa_K1970001 XOR gate like repressor of CI repressor expression 2016-10-13T11:00:00Z 2016-10-21T08:10:29Z De novo DNA. This part uses deoxyribozyme ribonucleases to degrade CI repressor mRNA, thereby silencing the gene. The deoxyribozyme ribonucleases are present in two copies, where expression of one is controlled by the lacI promoter and the other by the tetR promoter. The regulatory system works like an XOR gate: Inducing the regulator with one, and only one, inducer results in silencing of CI mRNA expression. Inducing both lacI and tetR promoters results in the deoxyribozyme ribonucleases binding to each other through designed complimentary regions, stopping their mRNA degrading activities. Lack of induction of either promoter should prevent either deoxyribozyme ribonuclease from being synthesized by the cell, in turn preventing their degradation of the CI repressor. false false _2437_ 20673 20673 9 false Designing the deoxyribozyme ribonucleases so that they bound more tightly to each other than to CI repressor mRNA was challenging. Additionally, choosing which promoters to place in front of either gene required some thought on desired expression levels and controlability of the system. false Eivind B??e Drejer annotation2514463 1 lacI promoter range2514463 1 1 200 annotation2514473 1 reverse transcriptase range2514473 1 1662 2624 annotation2514467 1 misc RNA range2514467 1 209 290 annotation2514464 1 DNAzyme range2514464 1 314 358 annotation2527136 1 misc RNA range2527136 1 1461 1542 annotation2514468 1 msDNA range2514468 1 1532 1641 annotation2514462 1 reverse transcriptase range2514462 1 410 1372 annotation2514472 1 DNAzyme range2514472 1 1566 1610 annotation2514466 1 tetR promoter range2514466 1 1399 1452 annotation2514465 1 msDNA range2514465 1 280 390 BBa_K1970001_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgacactcccaggacgcactcgttgtagctagcctcgccttaagcccgaggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaatactagtagcggccgctgcactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgactcgggcaggacgcactcgttgtagctagcctcgccttaagggagtggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z