LOCUS       BBa_K1970002            1874 bp    DNA     linear       29-Jun-2024
DEFINITION  Red fluorescent protein controlled by CI-repressor
ACCESSION   BBa_K1970002
VERSION     BBa_K1970002.1  
FEATURES             Location/Qualifiers
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     stem_loop       804..823
                     /label=T7 TE
     misc_feature    830..830
                     /label=stop
     polyA_site      824..837
                     /label=polya
     misc_feature    797..837
                     /label=BBa_B0012
     terminator      797..837
                     /label=BBa_B0012
     promoter        937..942
                     /label=-10
     promoter        914..919
                     /label=-35
     misc_feature    924..940
                     /label=OR1
     misc_feature    900..916
                     /label=OR2
     misc_feature    900..948
                     /label=BBa_R0051
     promoter        900..948
                     /label=BBa_R0051
     misc_feature    1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     misc_feature    44..55
                     /label=BBa_B0034
     CDS             62..775
                     /label=cIts
     misc_feature    62..775
                     /label=BBa_K098997
     misc_feature    797..837
                     /label=BBa_B0012
     stem_loop       804..823
                     /label=T7 TE
     polyA_site      824..837
                     /label=polya
     misc_feature    830..830
                     /label=stop
     misc_feature    846..891
                     /label=BBa_B0011
     stem_loop       858..880
                     /label=stem_loop
     misc_feature    900..916
                     /label=OR2
     misc_feature    900..948
                     /label=BBa_R0051
     promoter        914..919
                     /label=-35
     misc_feature    924..940
                     /label=OR1
     promoter        937..942
                     /label=-10
     misc_feature    776..788
                     /label=conserved
     misc_feature    1..948
                     /label=BBa_K608351
     promoter        994..999
                     /label=-10
     promoter        971..976
                     /label=-35
     misc_feature    981..997
                     /label=OR1
     misc_feature    957..973
                     /label=OR2
     misc_feature    957..1005
                     /label=BBa_R0051
     promoter        957..1005
                     /label=BBa_R0051
     misc_feature    1018..1021
                     /label=conserved
     RBS             1014..1025
                     /label=BBa_B0034
     CDS             1032..1706
                     /label=mrfp1
     misc_feature    1713..1737
                     /label=Help:Barcodes
     CDS             1032..1737
                     /label=BBa_E1010
     stem_loop       1757..1800
                     /label=stem_loop
     misc_feature    1746..1825
                     /label=BBa_B0010
     terminator      1746..1825
                     /label=BBa_B0010
     stem_loop       1841..1860
                     /label=T7 TE
     misc_feature    1867..1867
                     /label=stop
     polyA_site      1861..1874
                     /label=polya
     misc_feature    1834..1874
                     /label=BBa_B0012
     terminator      1834..1874
                     /label=BBa_B0012
     terminator      1746..1874
                     /label=BBa_B0015
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgactcca
      781 gcggccgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      841 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt ttactagagt
      901 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagtaac
      961 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta gagaaagagg
     1021 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc
     1081 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
     1141 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
     1201 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
     1261 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
     1321 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
     1381 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
     1441 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc
     1501 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
     1561 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
     1621 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
     1681 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac
     1741 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1801 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1861 tttctgcgtt tata
//