BBa_K1970003
1
BBa_K1970003
Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA
2016-10-16T11:00:00Z
2016-10-21T08:05:11Z
De novo DNA.
This gene, which is placed under the control of lacI, is transcribed into a particular type of single stranded DNA called deoxynucleotide ribonuclease. It targets the mRNA of the CI repressor protein, cleaving it after binding. This results in reduced or completely eliminated expression of the CI repressor gene, depending on the strength of the promoter used to control expression.
This gene is designed to be used in conjunction with another ribonuclease which targets and degrades CI repressor mRNA. The two genes target different parts of the mRNA. If expressed together, the ribonucleases bind to each other with higher affinity than to the CI repressor mRNA, in theory stopping the degradation of CI repressor mRNA.
Designed to work in conjunction with BBa_K1970002 for XOR gate like repression of CI repressor mRNA.
false
false
_2437_
20673
20673
9
false
Designing recognition sequences that would bind to CI repressor mRNA with high affinity, but to the gene product of BBa_K1970002 with even greater affinity was very challenging.
false
Eivind B??e Drejer
annotation2514494
1
DNAzyme
range2514494
1
168
212
annotation2514495
1
reverse transcriptase
range2514495
1
264
1226
annotation2514492
1
tetR promoter
range2514492
1
1
54
annotation2527134
1
misc RNA
range2527134
1
63
144
annotation2514493
1
msDNA
range2514493
1
134
243
BBa_K1970003_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgactcgggcaggacgcactcgttgtagctagcctcgccttaagggagtggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z