BBa_K1970003 1 BBa_K1970003 Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA 2016-10-16T11:00:00Z 2016-10-21T08:05:11Z De novo DNA. This gene, which is placed under the control of lacI, is transcribed into a particular type of single stranded DNA called deoxynucleotide ribonuclease. It targets the mRNA of the CI repressor protein, cleaving it after binding. This results in reduced or completely eliminated expression of the CI repressor gene, depending on the strength of the promoter used to control expression. This gene is designed to be used in conjunction with another ribonuclease which targets and degrades CI repressor mRNA. The two genes target different parts of the mRNA. If expressed together, the ribonucleases bind to each other with higher affinity than to the CI repressor mRNA, in theory stopping the degradation of CI repressor mRNA. Designed to work in conjunction with BBa_K1970002 for XOR gate like repression of CI repressor mRNA. false false _2437_ 20673 20673 9 false Designing recognition sequences that would bind to CI repressor mRNA with high affinity, but to the gene product of BBa_K1970002 with even greater affinity was very challenging. false Eivind B??e Drejer annotation2514495 1 reverse transcriptase range2514495 1 264 1226 annotation2514492 1 tetR promoter range2514492 1 1 54 annotation2527134 1 misc RNA range2527134 1 63 144 annotation2514493 1 msDNA range2514493 1 134 243 annotation2514494 1 DNAzyme range2514494 1 168 212 BBa_K1970003_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgactcgggcaggacgcactcgttgtagctagcctcgccttaagggagtggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z