BBa_K1970004
1
BBa_K1970004
Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA.
2016-10-16T11:00:00Z
2016-10-27T07:17:16Z
De novo DNA.
This gene, which is placed under the control of lacI, is transcribed into a particular type of single stranded DNA called deoxynucleotide ribonuclease. It targets the mRNA of the CI repressor protein, cleaving it after binding. This results in reduced or completely eliminated expression of the CI repressor gene, depending on the strength of the promoter used to control expression.
This gene is designed to be used in conjunction with another ribonuclease which targets and degrades CI repressor mRNA. The two genes target different parts of the mRNA. If expressed together, the ribonucleases bind to each other with higher affinity than to the CI repressor mRNA, in theory stopping the degradation of CI repressor mRNA.
false
false
_2437_
30065
20673
9
false
Designing the sequence such that the binding affinity to the CI repressor mRNA was high enough was challenging.
false
Eivind B??e Drejer
annotation2514458
1
reverse transcriptase
range2514458
1
410
1372
annotation2527135
1
misc RNA
range2527135
1
209
290
annotation2514459
1
lacI promoter
range2514459
1
1
200
annotation2514460
1
msDNA
range2514460
1
280
390
annotation2514461
1
DNAzyme
range2514461
1
314
358
BBa_K1970004_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagatgcgcacccttagcgagaggtttatcattaaggtcaacctctggatgttgtttcggcatcctgcattgaatctgagttactgtctgttttcctgaattgtcgacactcccaggacgcactcgttgtagctagcctcgccttaagcccgaggtaccaattcaggaaacccgttttttctgacgtaagggtgcgcaactttcatgaaatccgctgaatatttgaacacttttagattgagaaatctcggcctacctgtcatgaacaatttgcatgacatgtctaaggcgactcgcatatctgttgaaacacttcggttgttaatctatacagctgattttcgctataggatctacactgtagaaaagaaaggcccagagaagagaatgagaaccatttaccaaccttctcgagaacttaaagccttacaaggatgggttctacgtaacattttagataaactgtcgtcatctcctttttctattggatttgaaaagcaccaatctattttgaataatgctaccccgcatattggggcaaactttatactgaatattgatttggaggattttttcccaagtttaactgctaacaaagtttttggagtgttccattctcttggttataatcgactaatatcttcagttttgacaaaaatatgttgttataaaaatctgctaccacaaggtgctccatcatcacctaaattagctaatctaatatgttctaaacttgattatcgtattcagggttatgcaggtagtcggggcttgatatatacgagatatgccgatgacctcaccttatctgcacagtctatgaaaaaggttgttaaagcacgtgattttttattttctataatcccaagtgaaggattggttattaactcaaaaaaaacttgtattagtgggcctcgtagtcagaggaaagttacaggtttagttatttcacaagagaaagttgggataggtagagaaaaatataaagaaattagagcaaagatacatcatatattttgcggtaagtcttctgagatagaacacgttaggggatggttgtcatttattttaagtgtggattcaaaaagccataggagattaataacttatattagcaaattagaaaaaaaatatggaaagaaccctttaaataaagcgaagacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z