BBa_K197002
1
BBa_K197002
{OprF AtD}
2009-10-20T11:00:00Z
2015-05-08T01:11:17Z
<pre>
Pool O09ig57 through O09ig78, assemble by PCA
PCR O09ig57/O09ig78 on PCA reaction (710, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig57 {?OprF AtD?}
----
O09ig57 PCA assembly of {?OprF AtD?} (B09ig57)
CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC
O09ig58 PCA assembly of {?OprF AtD?} (B09ig57)
AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT
O09ig59 PCA assembly of {?OprF AtD?} (B09ig57)
GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA
O09ig60 PCA assembly of {?OprF AtD?} (B09ig57)
TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG
O09ig61 PCA assembly of {?OprF AtD?} (B09ig57)
TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC
O09ig62 PCA assembly of {?OprF AtD?} (B09ig57)
ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA
O09ig63 PCA assembly of {?OprF AtD?} (B09ig57)
TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC
O09ig64 PCA assembly of {?OprF AtD?} (B09ig57)
GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG
O09ig65 PCA assembly of {?OprF AtD?} (B09ig57)
CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA
O09ig66 PCA assembly of {?OprF AtD?} (B09ig57)
GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC
O09ig67 PCA assembly of {?OprF AtD?} (B09ig57)
CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC
O09ig68 PCA assembly of {?OprF AtD?} (B09ig57)
GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG
O09ig69 PCA assembly of {?OprF AtD?} (B09ig57)
CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG
O09ig70 PCA assembly of {?OprF AtD?} (B09ig57)
AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA
O09ig71 PCA assembly of {?OprF AtD?} (B09ig57)
ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA
O09ig72 PCA assembly of {?OprF AtD?} (B09ig57)
TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG
O09ig73 PCA assembly of {?OprF AtD?} (B09ig57)
CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA
O09ig74 PCA assembly of {?OprF AtD?} (B09ig57)
GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA
O09ig75 PCA assembly of {?OprF AtD?} (B09ig57)
GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG
O09ig76 PCA assembly of {?OprF AtD?} (B09ig57)
GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC
O09ig77 PCA assembly of {?OprF AtD?} (B09ig57)
TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA
O09ig78 PCA assembly of {?OprF AtD?} (B09ig57)
CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC
</pre>
This is an 8-stranded beta barrel, outermembrane protein. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.
false
false
_294_
0
4867
271
It's complicated
false
N/A
false
Patrick Harrigan
BBa_K197002_sequence
1
gatctgactctaaaatgactggcgcttacggtcagatcgaaaaaggtatctctgactctacctctgttggtctgatgctgggtggtaacaacaacaaagttgacatcgctacctctgacctgaaaggtaactctgcttacctgggtggttacgttaaacacgacaaaggtgctttccgtggtatcgctggtatcggtatccagtactctgactgggactctcaccgtaacaccatgtctgactcttacaaagaaaactactctgaccgtggtctgaacatctacgcagagggtaaatacaccaaagaactgaccgaaggtctgttcctggaaccgaaagttggtgtttcttacgactacatcaaacaggaatctatcaacgaatctaaagacaaagctctggctctggaagttgacggtaaagacttcgacaccctgtctggtaacgttggtctggacctgcgtaaagaaatcgttaccgaaaaaggtaaacacaacctgaccgctggtgttaactacacccgtatcctgtctggtgctgacgaagacaacctgactggcaacttcggtggtaactctttcgatattctggttccgcagaaaaccaaagacaacgtttctgttggtatcaaatacgacatcgaactggaaactggcgttatgctgggtgctaaagtttcttacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z