BBa_K197003 1 BBa_K197003 {cl02365 AtD} 2009-10-20T11:00:00Z 2015-05-08T01:11:17Z <pre> Pool O09ig79 through O09ig112, assemble by PCA PCR O09ig79/O09ig112 on PCA reaction (854, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig79 {?cl02365 AtD?} ---- O09ig79 PCA assembly of {?cl02365 AtD?} (B09ig79) CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA O09ig80 PCA assembly of {?cl02365 AtD?} (B09ig79) CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA O09ig81 PCA assembly of {?cl02365 AtD?} (B09ig79) ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT O09ig82 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT O09ig83 PCA assembly of {?cl02365 AtD?} (B09ig79) GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT O09ig84 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC O09ig85 PCA assembly of {?cl02365 AtD?} (B09ig79) AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT O09ig86 PCA assembly of {?cl02365 AtD?} (B09ig79) ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG O09ig87 PCA assembly of {?cl02365 AtD?} (B09ig79) GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT O09ig88 PCA assembly of {?cl02365 AtD?} (B09ig79) CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG O09ig89 PCA assembly of {?cl02365 AtD?} (B09ig79) CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT O09ig90 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC O09ig91 PCA assembly of {?cl02365 AtD?} (B09ig79) ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC O09ig92 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT O09ig93 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC O09ig94 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT O09ig95 PCA assembly of {?cl02365 AtD?} (B09ig79) GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA O09ig96 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT O09ig97 PCA assembly of {?cl02365 AtD?} (B09ig79) ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG O09ig98 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA O09ig99 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC O09ig100 PCA assembly of {?cl02365 AtD?} (B09ig79) CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT O09ig101 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC O09ig102 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA O09ig103 PCA assembly of {?cl02365 AtD?} (B09ig79) ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG O09ig104 PCA assembly of {?cl02365 AtD?} (B09ig79) TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA O09ig105 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC O09ig106 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG O09ig107 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA O09ig108 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC O09ig109 PCA assembly of {?cl02365 AtD?} (B09ig79) AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT O09ig110 PCA assembly of {?cl02365 AtD?} (B09ig79) CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA O09ig111 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC O09ig112 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT </pre> This is an Autotransporter of the AT-1 family from the organism Neisseria meningitidis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 4867 271 It's complicated false N/A false Patrick Harrigan BBa_K197003_sequence 1 gatctgaaaactctaacgctcgtatgtggatgactggcgttggtcagtggggtaacctggactacggttactctaacatggacgttgacttctacgctggtctggttggtgctgaaatcgacgttaccgaaaacaccaaagctggtctgttcttcggtgctggttctacccactacaaagctcacgaatacggtaaaatcgactctaacgacctccatgttggtctgtacggtgtttctaacatcaaagacgttgctgctgttaactacggtatcacccacaccaaccaggaccgtgacgctaaacgtaccctgtgggttggtcagacctctggttacaactctacctcttacgacgctaaaatcacccagttcttcctggaaggtgcttacacccaactcaacaacgacaaatactctatcgaaccgtacgctggtttcaactggctccatgtttctaccgactctatcaacgaacaggttggtaacatgcagttctctaccaaaaccgacaaccaggacatccaggttggcactatcggtctgcgtggtggttacccgttcaccgctggttctatcaacatggctctgaaaggtgacgtttctgcttctcacctgttcggtgacaaccgtccggaagctcaactctacctgtctaactctggtgaagctaccctgcgtggtggtaaactggacaacctgttcggtgttggtctgggtgttgacgctcaactcaacaaatctaccaccttcggtgtttcttaccagggtcagtacaactctgacgtttcttcttctggtatcaacgctaccctgaaagttaacttcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z