BBa_K197003
1
BBa_K197003
{cl02365 AtD}
2009-10-20T11:00:00Z
2015-05-08T01:11:17Z
<pre>
Pool O09ig79 through O09ig112, assemble by PCA
PCR O09ig79/O09ig112 on PCA reaction (854, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig79 {?cl02365 AtD?}
----
O09ig79 PCA assembly of {?cl02365 AtD?} (B09ig79)
CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA
O09ig80 PCA assembly of {?cl02365 AtD?} (B09ig79)
CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA
O09ig81 PCA assembly of {?cl02365 AtD?} (B09ig79)
ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT
O09ig82 PCA assembly of {?cl02365 AtD?} (B09ig79)
CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT
O09ig83 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT
O09ig84 PCA assembly of {?cl02365 AtD?} (B09ig79)
CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC
O09ig85 PCA assembly of {?cl02365 AtD?} (B09ig79)
AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT
O09ig86 PCA assembly of {?cl02365 AtD?} (B09ig79)
ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG
O09ig87 PCA assembly of {?cl02365 AtD?} (B09ig79)
GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT
O09ig88 PCA assembly of {?cl02365 AtD?} (B09ig79)
CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG
O09ig89 PCA assembly of {?cl02365 AtD?} (B09ig79)
CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT
O09ig90 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC
O09ig91 PCA assembly of {?cl02365 AtD?} (B09ig79)
ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC
O09ig92 PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT
O09ig93 PCA assembly of {?cl02365 AtD?} (B09ig79)
TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC
O09ig94 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT
O09ig95 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA
O09ig96 PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT
O09ig97 PCA assembly of {?cl02365 AtD?} (B09ig79)
ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG
O09ig98 PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA
O09ig99 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC
O09ig100 PCA assembly of {?cl02365 AtD?} (B09ig79)
CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT
O09ig101 PCA assembly of {?cl02365 AtD?} (B09ig79)
TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC
O09ig102 PCA assembly of {?cl02365 AtD?} (B09ig79)
GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA
O09ig103 PCA assembly of {?cl02365 AtD?} (B09ig79)
ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG
O09ig104 PCA assembly of {?cl02365 AtD?} (B09ig79)
TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA
O09ig105 PCA assembly of {?cl02365 AtD?} (B09ig79)
CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC
O09ig106 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG
O09ig107 PCA assembly of {?cl02365 AtD?} (B09ig79)
TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA
O09ig108 PCA assembly of {?cl02365 AtD?} (B09ig79)
GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC
O09ig109 PCA assembly of {?cl02365 AtD?} (B09ig79)
AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT
O09ig110 PCA assembly of {?cl02365 AtD?} (B09ig79)
CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA
O09ig111 PCA assembly of {?cl02365 AtD?} (B09ig79)
TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC
O09ig112 PCA assembly of {?cl02365 AtD?} (B09ig79)
CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT
</pre>
This is an Autotransporter of the AT-1 family from the organism Neisseria meningitidis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.
false
false
_294_
0
4867
271
It's complicated
false
N/A
false
Patrick Harrigan
BBa_K197003_sequence
1
gatctgaaaactctaacgctcgtatgtggatgactggcgttggtcagtggggtaacctggactacggttactctaacatggacgttgacttctacgctggtctggttggtgctgaaatcgacgttaccgaaaacaccaaagctggtctgttcttcggtgctggttctacccactacaaagctcacgaatacggtaaaatcgactctaacgacctccatgttggtctgtacggtgtttctaacatcaaagacgttgctgctgttaactacggtatcacccacaccaaccaggaccgtgacgctaaacgtaccctgtgggttggtcagacctctggttacaactctacctcttacgacgctaaaatcacccagttcttcctggaaggtgcttacacccaactcaacaacgacaaatactctatcgaaccgtacgctggtttcaactggctccatgtttctaccgactctatcaacgaacaggttggtaacatgcagttctctaccaaaaccgacaaccaggacatccaggttggcactatcggtctgcgtggtggttacccgttcaccgctggttctatcaacatggctctgaaaggtgacgtttctgcttctcacctgttcggtgacaaccgtccggaagctcaactctacctgtctaactctggtgaagctaccctgcgtggtggtaaactggacaacctgttcggtgttggtctgggtgttgacgctcaactcaacaaatctaccaccttcggtgtttcttaccagggtcagtacaactctgacgtttcttcttctggtatcaacgctaccctgaaagttaacttcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z