BBa_K197004
1
BBa_K197004
{VtaA11 AtD}
2009-10-20T11:00:00Z
2015-05-08T01:11:18Z
<pre>
Pool O09ig113 through O09ig120, assemble by PCA
PCR O09ig113/O09ig120 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig113 {?VtaA11 AtD?}
----
O09ig113 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG
O09ig114 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT
O09ig115 PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT
O09ig116 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA
O09ig117 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA
O09ig118 PCA assembly of {?VtaA11 AtD?} (B09ig113)
TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA
O09ig119 PCA assembly of {?VtaA11 AtD?} (B09ig113)
ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG
O09ig120 PCA assembly of {?VtaA11 AtD?} (B09ig113)
CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC
</pre>
This is an Autotransporter of the AT-2 family from the organism Haemophilus parasuis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.
false
false
_294_
0
4867
271
It's complicated
false
N/A
false
Patrick Harrigan
BBa_K197004_sequence
1
gatctaacaacaaagctctgcgtgctggtatcgctggttctaacgctgctgctggtctgccgcaggtttatattccgggtaaatctatggttgctgtttctgctggcactttcaaaggtcagtctgctctggctgttggttactctcgtgcttctgacaacggtaaactgatcctgaaactccaaggtaacgctaacacctctggtgaaatgggtggttctgttggtgttggttaccagtggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z