BBa_K197005 1 BBa_K197005 {Hag AtD} 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z <pre> Pool O09ig121 through O09ig128, assemble by PCA PCR O09ig121/O09ig128 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig121 {?Hag AtD?} ---- O09ig121 PCA assembly of {?Hag AtD?} (B09ig121) CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT O09ig122 PCA assembly of {?Hag AtD?} (B09ig121) CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG O09ig123 PCA assembly of {?Hag AtD?} (B09ig121) TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG O09ig124 PCA assembly of {?Hag AtD?} (B09ig121) CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA O09ig125 PCA assembly of {?Hag AtD?} (B09ig121) CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC O09ig126 PCA assembly of {?Hag AtD?} (B09ig121) TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA O09ig127 PCA assembly of {?Hag AtD?} (B09ig121) AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG O09ig128 PCA assembly of {?Hag AtD?} (B09ig121) CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT </pre> This is a 200kDa protein with 10-stranded beta barrel. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 4867 271 It's complicated false N/A false Patrick Harrigan BBa_K197005_sequence 1 gatctaacgaaaacaaagctaacgctggtatctcttctgctatggctatggcttctatgccgcaggcttatattccgggtcgttctatggttactggcggtatcgctacccacaacggtcagggtgctgttgctgttggtctgtctaaactgtctgacaacggtcagtgggttttcaaaatcaacggttctgctgacactcaaggccatgtaggtgctgctgttggtgctggtttccacttcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z