BBa_K197007
1
BBa_K197007
{Hia AtD}
2009-10-20T11:00:00Z
2015-05-08T01:11:18Z
<pre>
Pool O09ig137 through O09ig144, assemble by PCA
PCR O09ig137/O09ig144 on PCA reaction (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig137 {?Hia AtD?}
----
O09ig137 PCA assembly of {?Hia AtD?} (B09ig137)
CCATAGAATTCATGAGATCTCTGCGTCGTGAAACCTCTGGTGGTGTTG
O09ig138 PCA assembly of {?Hia AtD?} (B09ig137)
CCTGCGGCAGGTTAGCGGTAGCGATAGCAGCAGCAACACCACCAGAGGTTTCA
O09ig139 PCA assembly of {?Hia AtD?} (B09ig137)
TACCGCTAACCTGCCGCAGGCTTACGTTCAGGGTCGTGGTATGACCTCTGTTG
O09ig140 PCA assembly of {?Hia AtD?} (B09ig137)
CGATAGCAGACTGACCCTGGTAAGAAGAAACACCAACAGAGGTCATACCACGA
O09ig141 PCA assembly of {?Hia AtD?} (B09ig137)
CCAGGGTCAGTCTGCTATCGCTGTTGGTGTTTCTGCTGTTTCTGAATCTGGTC
O09ig142 PCA assembly of {?Hia AtD?} (B09ig137)
TGTTAGCAGAACCAGAGAATTTGAAAACCCAGTGACCAGATTCAGAAACAGCA
O09ig143 PCA assembly of {?Hia AtD?} (B09ig137)
ATTCTCTGGTTCTGCTAACACCCGTTCTCACGTTGGTGTTGGTGCTGGTGTTG
O09ig144 PCA assembly of {?Hia AtD?} (B09ig137)
CGTTAGGATCCCCATTGATAGCCAACACCAGCACCAACACCA
</pre>
Autotransporter from Haemophilus influenzae. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.
false
false
_294_
0
4867
271
It's complicated
false
N/A
false
Patrick Harrigan
BBa_K197007_sequence
1
gatctctgcgtcgtgaaacctctggtggtgttgctgctgctatcgctaccgctaacctgccgcaggcttacgttcagggtcgtggtatgacctctgttggtgtttcttcttaccagggtcagtctgctatcgctgttggtgtttctgctgtttctgaatctggtcactgggttttcaaattctctggttctgctaacacccgttctcacgttggtgttggtgctggtgttggctatcaatggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z