BBa_K197010 1 BBa_K197010 {ehaB} 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z <pre> PCR ca998/O09ig281 on M10052 (1229, EcoRI/BamHI) Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-O09ig281 {<ehaB>} ------------------------------------- Ca998 forward oligo gtatcacgaggcagaatttcag O09ig281 reverse oligo with stop codons removed for {<ehaB>} cggtaGGATCCccaggtatatttaacaccca </pre> The primary sequence alone is sufficient for crossing the bacterial membrane. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 5247 9 It's complicated false N/A false Gabriela Guzman Lopez Aguado BBa_K197010_sequence 1 gatctggcttaatcgcaatgaactctgatggtcacctgactattaacggtaacggcgataacgacaacactgcttctatcgaagctggtcagaacgaagttgataacaacggtgaccatgttgcagccgcgaccggtaactacaaagttcgtatcgacaacgctactggtgctggttctatcgctgactacaacggcaacgagctgatctacgtcaacgacaaaaacagcaacgcgaccttctctgctgctaacaaagctgacctgggtgcatacacctatcaggctgaacagcgcggtaacaccgttgttctgcaacagatggagttgaccgactacgctaacatggcgctgagcatcccatctgcgaacaccaatatctggaacctggaacaagacaccgttggtactcgtttgaccaactctcgtcatggcctggctgataacggcggcgcatgggtaagctacttcggtggtaacttcaacggcgacaacggcaccatcaactatgatcaggatgttaacggcatcatggtcggtgttgataccaaaattgacggtaacaacgctaagtggatcgtcggtgcggctgcaggcttcgctaaaggtgacatgaatgaccgttctggtcaggtggatcaagacagccagactgcctacatctactcttctgctcacttcgcgaacaacgtctttgttgatggtagcttgagctactctcacttcaacaacgacctgtctgcaaccatgagcaacggtacttacgttgacggtagcaccaactccgacgcttggggcttcggtttgaaagccggttacgacttcaaactgggcgatgctggttacgtgactccttacggcagcatttctggtctgttccagtctggtgatgactaccagctgagcaacgacatgaaagttgacggtcagtcttacgacagcatgcgttatgaactgggtgtagatgcaggttataccttcacctacagcgaagatcaggctctgactccgtacttcaaactggcttacgtctacgacgactctaacaacgataacgatgtgaacggtgattccatcgataacggtactgaagggtctgcggtacgtgttggtctgggtactcagttcagcttcaccaagaacttcagcgcctataccgatgctaactacctcggtggtggtgacgtagatcaagactggtccgcgaacgtgggtgttaaatatacctggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z