BBa_K197013 1 BBa_K197013 {YuaQ AtD (putative)} 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z <pre> PCR O09ig306/O09ig305 on MG1655(ask chris about EcoRV before start) (464bp, gp = frag1) PCR O09ig304/O09ig307 on MG1655(ask chris about EcoRV before start) (452bp, gp = frag2) ---- PCR O09ig306/O09ig307 on mixed frags (898bp, EcoRI/BamHI) Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig306 {?yuaQ AtD?} ---- O09ig306 Forward PCR of {?yuaQ AtD?} (B09ig306) AAGTGGAATTCATGAGATCTGGAGAACCGAAAGCAACCAG O09ig305 Reverse internal oligo for {?yuaQ AtD?} (B09ig306) GCTGCTGAACTCCCCGATTTTC O09ig304 Forward internal oligo for {?yuaQ AtD?} (B09ig306) GAAAATCGGGGAGTTCAGCAGC O09ig307 Reverse PCR of {?yuaQ AtD?} (B09ig306) GTTAGGGATCCGAATGTATACTTCACACCCAG </pre> Features: bears sequence similarity to the confirmed autotransporters AIDA and Ag43. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 5247 9 It's complicated false N/A false Gabriela Guzman Lopez Aguado BBa_K197013_sequence 1 gatctggagaaccgaaagcaaccagtatgtggatgcgccatgaaggaggacataaccgctggcgcgacggttctggtcaactgaaaactcaaagtaatcgttatgtgattcaactgggtggtgatatcgcacaatgggattggggtggaactaaccgctggcatctgggggttatggctggttacggtaacaaccatagcagtacaggtgcagtacgcaccggataccattcaaaaggcagcgtaaatggttacagtaccgggctgtatgctacctggtatgctgatgatgagactcataacggagcttatctggatacctgggctcagtacggctggttcgataaccatgtgaaaggtgacggactgccgggtgaatcctggaaatcaaaaggacttaccgcctcactggaaacaggttatgcctggaaaatcggggagttcagcagcaactatggcaatctgaacgaatggtatgttcagcctcaggcccagcttgtctggatgggggttaaagctgatgaactctatgaaagtaacggtactcttattgaaagcaccggagacggtaatgtccatacccgtctgggcgtgaaaacctggattaaaaggctcaataaaatggatgatggtaaatcacgtgagttcagcccatttgtggaggtgaactggttgcataatacccgtgattttggggttcgtatgaacggcgaaccggtgtatcaggatggtacccgtaatatcggagaggtcaaaactggtgtggaaggtcaaataaatccccatctgaacctgtgggggaatgtgagagttcaagttggtgataaagggtataacgacacctcagccatgctgggtgtgaagtatacattcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z