BBa_K197015
1
BBa_K197015
{eCPX (circularly permuted OmpX)}
2009-10-20T11:00:00Z
2015-05-08T01:11:18Z
<pre>
PCR ca998/O09ig282 on M10014 (477, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig282 {<eCPX>}
-------------------------------------
Ca998 forward oligo
gtatcacgaggcagaatttcag
O09ig282 reverse oligo with stop codons removed for {<eCPX>}
ccggtGGATCCAGAGCTTGCAGTACGGCTTT
</pre>
Features: protein is an enhanced CPX variant located in the outermembrane that joins the N- and C-termini of OmpX. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.
false
false
_294_
0
5247
9
It's complicated
false
N/A
false
Gabriela Guzman Lopez Aguado
BBa_K197015_sequence
1
gatctggtcagtctggtgactacaacaaaaaccagtactacggcatcactgctggtccggcttaccgcattaacgactgggcaagcatctacggtgtagtgggtgtgggttatggtaaattccagaccactgaatacccgacctacaaacacgacaccagcgactacggtttctcctacggtgcgggtctgcagttcaacccgatggaaaacgttgctctggacttctcttacgagcagagccgtattcgtagcgttgacgtaggcacctggattgcctctgttggttaccgcttcggatctaaacagtctaaagcgacttctactgtaactggcggttacgcacagagcgacgctcagggccaaatgaacaaaatgggcggtttcaacctgaaataccgctatgaagaagacaacagcccgctgggtgtgatcggttctttcacttacaccgagaaaagccgtactgcaagctctg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z