LOCUS       BBa_K197032             2712 bp    DNA     linear       28-Jun-2024
DEFINITION  {Pbad.rbs.prepro.StrepTag}{type IIIs Needle Complex 
            scFV}{upaG_short}(dblterm)
ACCESSION   BBa_K197032
VERSION     BBa_K197032.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     CDS             1461..2297
                     /label=BBa_K197019
     CDS             2306..2575
                     /label=BBa_K197008
     stem_loop       2595..2638
                     /label=stem_loop
     misc_feature    2584..2663
                     /label=BBa_B0010
     terminator      2584..2663
                     /label=BBa_B0010
     stem_loop       2679..2698
                     /label=T7 TE
     misc_feature    2705..2705
                     /label=stop
     polyA_site      2699..2712
                     /label=polya
     misc_feature    2672..2712
                     /label=BBa_B0012
     terminator      2672..2712
                     /label=BBa_B0012
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagag gatctgctga actggttatg acccagaccc cgtctccggt
     1501 ttccgctgct gttggtggca ctgttaccat ctcttgccaa tcctctcagt ctgtttacaa
     1561 caacaacctg ctgtcttggt atcagcagaa accgggtcag ccgccgaaac tgctgatcta
     1621 caacgcttct aaactggctt ctggtgttcc gtctcgtttc aaaggttctg gttctggcac
     1681 tcagttcacc ctgactattt ccgatctcga atgcgacgac gcagcaacct actactgcca
     1741 gggcacttac tactcttctg gttggtatct gaccttcggt ggtggcacta aactggacgt
     1801 taaaggtcag tctggtcagt ctggtaaacc gggttctggt gaaggtcagg gtgttggttc
     1861 tcagcaacaa ctcgttgaat ctggtggtcg tctggttact ccaggcactc cgctgaccct
     1921 gcgttgcacc gcttctggtt tctctctgtc taactactac atgaactggg ttcgtcaggc
     1981 tccgggtaaa ggtctggaat ggatcggtat catctacgaa tctggtttca cctactacgc
     2041 ttcttgggct aaaggtcgtt tcaccatctc taaaacctct accactatgg acctgaaaat
     2101 cacctcccca accaccgagg ataccgctac ctacttctgc gctcgtttcg acatcggtta
     2161 ccgtggtggt gctttcgacc cgtggggtcc gggcactctg gttaccgttt cttctggtca
     2221 gccgaaagct ccgtctgttt ctcaactcgt tggtgctgaa caaaagctca tctccgaaga
     2281 ggacctgaac ggtgctgtac tagaggatct gttgagatgg ataacaaact gtccaaaact
     2341 gaaagcaagc tgagtggtgg tatcgcttct gcaatggcaa tgaccggtct gccgcaggct
     2401 tacacgccgg gtgccagcat ggcctctatt ggtggcggta cttacaacgg tgaatcggct
     2461 gttgctttag gtgtgtcgat ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt
     2521 agtaccaata gccagggtga atactccgcc gcactcggtg ccggtattca gtgggtacta
     2581 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2641 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2701 tctgcgttta ta
//