BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_M10093 1 BBa_M10093 {Pbad.rbs.prepro.StrepTag&#707;} 2009-04-26T11:00:00Z 2015-05-08T01:13:51Z PCR from synthesized DNAs Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar. false false _271_ 0 483 271 Not in stock false N/A false John Anderson annotation2002590 1 Pbad range2002590 1 956 1193 annotation2002591 1 PrePro Sequence range2002591 1 1290 1358 annotation2002592 1 Streptavidin Binding Peptide range2002592 1 1374 1412 annotation2002593 1 rbs range2002593 1 1274 1289 annotation2002589 1 AraC range2002589 1 50 805 BBa_K197032 1 BBa_K197032 {Pbad.rbs.prepro.StrepTag}{type IIIs Needle Complex scFV}{upaG_short}(dblterm) 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z BglBrick assembly This is a cell surface display device that exposes a Type III Needle scFv to the extracellular environment via fusion to the autotransporter upaG_short. The Type III needle scFv will bind to the needle complex present on the surface of pathogenic E.coli. This device will enable engineered E.coli to bind preferentially to pathogenic E.coli. It can be used in projects that require pathogen detection. false false _294_ 0 4745 271 It's complicated true N/A false Jennifer Brophy component2062179 1 BBa_K197008 component2062182 1 BBa_B0012 component2062180 1 BBa_B0010 component2062178 1 BBa_K197019 component2062177 1 BBa_M10093 annotation2062182 1 BBa_B0012 range2062182 1 2672 2712 annotation2062177 1 BBa_M10093 range2062177 1 1 1452 annotation2062178 1 BBa_K197019 range2062178 1 1461 2297 annotation2062179 1 BBa_K197008 range2062179 1 2306 2575 annotation2062180 1 BBa_B0010 range2062180 1 2584 2663 BBa_K197019 1 BBa_K197019 TypeIII Needle scFv 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z gene synthesized, sequence taken from La Ragione, R et al. Recombinant anti-EspA antibodies block Escherichia coli O157:H7-induced attaching and effacing lesions in vitro. Microbes and Infection. September 2005; 8: 426???433. Available Online: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VPN-4H4T215-1-7&_cdi=6211&_user=4420&_orig=search&_coverDate=02%2F28%2F2006&_sk=999919997&view=c&wchp=dGLbVtz-zSkzS&md5=f35699db5dcd202f4cd1c1d8739d89b0&ie=/sdarticle.pdf This antibody detects the espA motif of the typeIII needle complex present in many pathogenic bacteria. This can be surface displayed for pathogen detection applciations. false false _294_ 0 5247 9 It's complicated true n/a false Gabriela Guzman Lopez Aguado BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K197008 1 BBa_K197008 {upaG_short} 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z <pre> PCR ca998/O09ig277 on M10026 (279, EcoRI/BamHI) Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09upaG {<espP(beta)>} ---- ca998 Forward Oligo CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC O09ig277 Reverse Oligo for the removal of stop codon from {<upaG_short>} CGTTAGGATCCccactgaataccggcaccgagtgcg </pre> An Autotransporter from Escherichia Coli. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 4867 271 It's complicated false N/A false Patrick Harrigan BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K197019_sequence 1 gatctgctgaactggttatgacccagaccccgtctccggtttccgctgctgttggtggcactgttaccatctcttgccaatcctctcagtctgtttacaacaacaacctgctgtcttggtatcagcagaaaccgggtcagccgccgaaactgctgatctacaacgcttctaaactggcttctggtgttccgtctcgtttcaaaggttctggttctggcactcagttcaccctgactatttccgatctcgaatgcgacgacgcagcaacctactactgccagggcacttactactcttctggttggtatctgaccttcggtggtggcactaaactggacgttaaaggtcagtctggtcagtctggtaaaccgggttctggtgaaggtcagggtgttggttctcagcaacaactcgttgaatctggtggtcgtctggttactccaggcactccgctgaccctgcgttgcaccgcttctggtttctctctgtctaactactacatgaactgggttcgtcaggctccgggtaaaggtctggaatggatcggtatcatctacgaatctggtttcacctactacgcttcttgggctaaaggtcgtttcaccatctctaaaacctctaccactatggacctgaaaatcacctccccaaccaccgaggataccgctacctacttctgcgctcgtttcgacatcggttaccgtggtggtgctttcgacccgtggggtccgggcactctggttaccgtttcttctggtcagccgaaagctccgtctgtttctcaactcgttggtgctgaacaaaagctcatctccgaagaggacctgaacggtgctg BBa_K197008_sequence 1 gatctgttgagatggataacaaactgtccaaaactgaaagcaagctgagtggtggtatcgcttctgcaatggcaatgaccggtctgccgcaggcttacacgccgggtgccagcatggcctctattggtggcggtacttacaacggtgaatcggctgttgctttaggtgtgtcgatggtgagcgccaatggtcgttgggtctacaaattacaaggtagtaccaatagccagggtgaatactccgccgcactcggtgccggtattcagtggg BBa_K197032_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagaggatctgctgaactggttatgacccagaccccgtctccggtttccgctgctgttggtggcactgttaccatctcttgccaatcctctcagtctgtttacaacaacaacctgctgtcttggtatcagcagaaaccgggtcagccgccgaaactgctgatctacaacgcttctaaactggcttctggtgttccgtctcgtttcaaaggttctggttctggcactcagttcaccctgactatttccgatctcgaatgcgacgacgcagcaacctactactgccagggcacttactactcttctggttggtatctgaccttcggtggtggcactaaactggacgttaaaggtcagtctggtcagtctggtaaaccgggttctggtgaaggtcagggtgttggttctcagcaacaactcgttgaatctggtggtcgtctggttactccaggcactccgctgaccctgcgttgcaccgcttctggtttctctctgtctaactactacatgaactgggttcgtcaggctccgggtaaaggtctggaatggatcggtatcatctacgaatctggtttcacctactacgcttcttgggctaaaggtcgtttcaccatctctaaaacctctaccactatggacctgaaaatcacctccccaaccaccgaggataccgctacctacttctgcgctcgtttcgacatcggttaccgtggtggtgctttcgacccgtggggtccgggcactctggttaccgtttcttctggtcagccgaaagctccgtctgtttctcaactcgttggtgctgaacaaaagctcatctccgaagaggacctgaacggtgctgtactagaggatctgttgagatggataacaaactgtccaaaactgaaagcaagctgagtggtggtatcgcttctgcaatggcaatgaccggtctgccgcaggcttacacgccgggtgccagcatggcctctattggtggcggtacttacaacggtgaatcggctgttgctttaggtgtgtcgatggtgagcgccaatggtcgttgggtctacaaattacaaggtagtaccaatagccagggtgaatactccgccgcactcggtgccggtattcagtgggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M10093_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z