LOCUS       BBa_K197033             2145 bp    DNA     linear       28-Jun-2024
DEFINITION  {Pbad.rbs.prepro.StrepTag}{INP repeats}{VtaA11 AtD}(dblterm)
ACCESSION   BBa_K197033
VERSION     BBa_K197033.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     CDS             1461..1757
                     /label=BBa_K197026
     CDS             1766..2008
                     /label=BBa_K197004
     stem_loop       2028..2071
                     /label=stem_loop
     misc_feature    2017..2096
                     /label=BBa_B0010
     terminator      2017..2096
                     /label=BBa_B0010
     stem_loop       2112..2131
                     /label=T7 TE
     misc_feature    2138..2138
                     /label=stop
     polyA_site      2132..2145
                     /label=polya
     misc_feature    2105..2145
                     /label=BBa_B0012
     terminator      2105..2145
                     /label=BBa_B0012
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagag aaccacagtg atctaattgc cggttatgga agtacaggca
     1501 ccgccggctc cgacagctgg ctggtcgctg gctatggaag cacccagacc gccggtgggg
     1561 acagcgcgct gacagcgggt tacggcagca cccagaccgc ccgcgaaggc agcaacctga
     1621 cggcagggta cggcagcacc ggcacggcag gctcggacag ttcgctgatc gccggttacg
     1681 gcagtactca gacttcgggc ggggacagct cactcactgc gggctacggc agcactgcaa
     1741 cggccgggca ggacagttac tagaggatct aacaacaaag ctctgcgtgc tggtatcgct
     1801 ggttctaacg ctgctgctgg tctgccgcag gtttatattc cgggtaaatc tatggttgct
     1861 gtttctgctg gcactttcaa aggtcagtct gctctggctg ttggttactc tcgtgcttct
     1921 gacaacggta aactgatcct gaaactccaa ggtaacgcta acacctctgg tgaaatgggt
     1981 ggttctgttg gtgttggtta ccagtgggta ctagagccag gcatcaaata aaacgaaagg
     2041 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2101 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//