LOCUS BBa_K197034 3419 bp DNA linear 26-Dec-2024 DEFINITION {Pbad.rbs.prepro.StrepTag}{mgfp-5}{GFP-LVA}{cl02365 AtD}(dblterm) ACCESSION BBa_K197034 VERSION BBa_K197034.1 FEATURES Location/Qualifiers CDS 50..805 /label=AraC promoter 956..1193 /label=Pbad CDS 1290..1358 /label=PrePro Sequence CDS 1374..1412 /label=Streptavidin Binding Peptide RBS 1274..1289 /label=rbs misc_feature 1..1452 /label=BBa_M10093 CDS 1461..1688 /label=BBa_K197018 CDS 1697..2446 /label=BBa_K197030 CDS 2455..3282 /label=BBa_K197003 stem_loop 3302..3345 /label=stem_loop misc_feature 3291..3370 /label=BBa_B0010 terminator 3291..3370 /label=BBa_B0010 stem_loop 3386..3405 /label=T7 TE misc_feature 3412..3412 /label=stop polyA_site 3406..3419 /label=polya misc_feature 3379..3419 /label=BBa_B0012 terminator 3379..3419 /label=BBa_B0012 ORIGIN 1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac 301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc 481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga 1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc 1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca 1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac 1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg 1441 cagtctgggc agtactagag tcttccgagg aatacaaagg tggttactac ccgggtaaca 1501 cctaccacta ccactctggt ggttcttacc acggttctgg ttaccacggt ggttacaaag 1561 gtaaatacta cggtaaagct aaaaaatact actacaaata caaaaactct ggtaaataca 1621 aatacctgaa aaaagctcgt aaataccacc gtaaaggtta caaaaaatac tacggtggtg 1681 gttcttctta ctagagagta aaggagaaga acttttcact ggagttgtcc caattcttgt 1741 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 1801 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 1861 atggccaaca cttgtcacta ctttgactta tggtgttcaa tgcttttcaa gatacccaga 1921 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 1981 aactatattt ttcaaagatg acgggaacta taagacacgt gctgaagtca agtttgaagg 2041 tgatacactt gttaatagaa tcgagttaaa gggtattgat tttaaagaag atggaaacat 2101 tcttggacac aagttggaat acaactataa ctcacacaat gtatacatca tggcagacaa 2161 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 2221 ccaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 2281 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 2341 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 2401 atacaaaagg cctgcagcaa acgacgaaaa ctacgcttta gtagcttact agaggatctg 2461 aaaactctaa cgctcgtatg tggatgactg gcgttggtca gtggggtaac ctggactacg 2521 gttactctaa catggacgtt gacttctacg ctggtctggt tggtgctgaa atcgacgtta 2581 ccgaaaacac caaagctggt ctgttcttcg gtgctggttc tacccactac aaagctcacg 2641 aatacggtaa aatcgactct aacgacctcc atgttggtct gtacggtgtt tctaacatca 2701 aagacgttgc tgctgttaac tacggtatca cccacaccaa ccaggaccgt gacgctaaac 2761 gtaccctgtg ggttggtcag acctctggtt acaactctac ctcttacgac gctaaaatca 2821 cccagttctt cctggaaggt gcttacaccc aactcaacaa cgacaaatac tctatcgaac 2881 cgtacgctgg tttcaactgg ctccatgttt ctaccgactc tatcaacgaa caggttggta 2941 acatgcagtt ctctaccaaa accgacaacc aggacatcca ggttggcact atcggtctgc 3001 gtggtggtta cccgttcacc gctggttcta tcaacatggc tctgaaaggt gacgtttctg 3061 cttctcacct gttcggtgac aaccgtccgg aagctcaact ctacctgtct aactctggtg 3121 aagctaccct gcgtggtggt aaactggaca acctgttcgg tgttggtctg ggtgttgacg 3181 ctcaactcaa caaatctacc accttcggtg tttcttacca gggtcagtac aactctgacg 3241 tttcttcttc tggtatcaac gctaccctga aagttaactt cgtactagag ccaggcatca 3301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3361 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //