BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K197018 1 BBa_K197018 mgfp-5 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z gene synthesized, gene taken from this paper: Dong Soo Hwang et al. Expression of Functional Recombinant Mussel Adhesive Protein Mgfp-5 in Eschericia coli. American Society for Microbiology. June 2004; 70(6): 3352-3359. Available online at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC427802/ A mussel foot protein that plays a role in the adhesion of the organism to rocks and other substrates. This can be surface displayed on bacterial cells to make an adherent bacteria or a glue like substance. false false _294_ 0 5247 9 It's complicated true n/a false Gabriela Guzman Lopez Aguado BBa_K197030 1 BBa_K197030 GFP-LVA 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z synthesis This is a spacer to be put between the displayer and passenger for e coli cell surface display false false _294_ 0 4737 271 It's complicated false N/A false John Wang BBa_K197034 1 BBa_K197034 {Pbad.rbs.prepro.StrepTag}{mgfp-5}{GFP-LVA}{cl02365 AtD}(dblterm) 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z BglBrick assembly This is a cell surface display device that exposes mgfp-5 (a mussel foot adhesion protein) to the extracellular environment via fusion to the putative autotransporter {cl02365 AtD}. When expressed in the correct environment mgfp-5 should form an underwater stable bio-glue. This device will enable engineered E.coli to bind to surfaces. false false _294_ 0 4745 271 It's complicated true N/A false Jennifer Brophy component2062161 1 BBa_M10093 component2062167 1 BBa_B0012 component2062165 1 BBa_B0010 component2062163 1 BBa_K197030 component2062162 1 BBa_K197018 component2062164 1 BBa_K197003 annotation2062165 1 BBa_B0010 range2062165 1 3291 3370 annotation2062167 1 BBa_B0012 range2062167 1 3379 3419 annotation2062161 1 BBa_M10093 range2062161 1 1 1452 annotation2062162 1 BBa_K197018 range2062162 1 1461 1688 annotation2062164 1 BBa_K197003 range2062164 1 2455 3282 annotation2062163 1 BBa_K197030 range2062163 1 1697 2446 BBa_K197003 1 BBa_K197003 {cl02365 AtD} 2009-10-20T11:00:00Z 2015-05-08T01:11:17Z <pre> Pool O09ig79 through O09ig112, assemble by PCA PCR O09ig79/O09ig112 on PCA reaction (854, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig79 {?cl02365 AtD?} ---- O09ig79 PCA assembly of {?cl02365 AtD?} (B09ig79) CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA O09ig80 PCA assembly of {?cl02365 AtD?} (B09ig79) CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA O09ig81 PCA assembly of {?cl02365 AtD?} (B09ig79) ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT O09ig82 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT O09ig83 PCA assembly of {?cl02365 AtD?} (B09ig79) GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT O09ig84 PCA assembly of {?cl02365 AtD?} (B09ig79) CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC O09ig85 PCA assembly of {?cl02365 AtD?} (B09ig79) AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT O09ig86 PCA assembly of {?cl02365 AtD?} (B09ig79) ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG O09ig87 PCA assembly of {?cl02365 AtD?} (B09ig79) GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT O09ig88 PCA assembly of {?cl02365 AtD?} (B09ig79) CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG O09ig89 PCA assembly of {?cl02365 AtD?} (B09ig79) CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT O09ig90 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC O09ig91 PCA assembly of {?cl02365 AtD?} (B09ig79) ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC O09ig92 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT O09ig93 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC O09ig94 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT O09ig95 PCA assembly of {?cl02365 AtD?} (B09ig79) GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA O09ig96 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT O09ig97 PCA assembly of {?cl02365 AtD?} (B09ig79) ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG O09ig98 PCA assembly of {?cl02365 AtD?} (B09ig79) GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA O09ig99 PCA assembly of {?cl02365 AtD?} (B09ig79) GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC O09ig100 PCA assembly of {?cl02365 AtD?} (B09ig79) CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT O09ig101 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC O09ig102 PCA assembly of {?cl02365 AtD?} (B09ig79) GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA O09ig103 PCA assembly of {?cl02365 AtD?} (B09ig79) ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG O09ig104 PCA assembly of {?cl02365 AtD?} (B09ig79) TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA O09ig105 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC O09ig106 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG O09ig107 PCA assembly of {?cl02365 AtD?} (B09ig79) TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA O09ig108 PCA assembly of {?cl02365 AtD?} (B09ig79) GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC O09ig109 PCA assembly of {?cl02365 AtD?} (B09ig79) AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT O09ig110 PCA assembly of {?cl02365 AtD?} (B09ig79) CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA O09ig111 PCA assembly of {?cl02365 AtD?} (B09ig79) TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC O09ig112 PCA assembly of {?cl02365 AtD?} (B09ig79) CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT </pre> This is an Autotransporter of the AT-1 family from the organism Neisseria meningitidis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 4867 271 It's complicated false N/A false Patrick Harrigan BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_M10093 1 BBa_M10093 {Pbad.rbs.prepro.StrepTag&#707;} 2009-04-26T11:00:00Z 2015-05-08T01:13:51Z PCR from synthesized DNAs Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar. false false _271_ 0 483 271 Not in stock false N/A false John Anderson annotation2002591 1 PrePro Sequence range2002591 1 1290 1358 annotation2002592 1 Streptavidin Binding Peptide range2002592 1 1374 1412 annotation2002593 1 rbs range2002593 1 1274 1289 annotation2002589 1 AraC range2002589 1 50 805 annotation2002590 1 Pbad range2002590 1 956 1193 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K197003_sequence 1 gatctgaaaactctaacgctcgtatgtggatgactggcgttggtcagtggggtaacctggactacggttactctaacatggacgttgacttctacgctggtctggttggtgctgaaatcgacgttaccgaaaacaccaaagctggtctgttcttcggtgctggttctacccactacaaagctcacgaatacggtaaaatcgactctaacgacctccatgttggtctgtacggtgtttctaacatcaaagacgttgctgctgttaactacggtatcacccacaccaaccaggaccgtgacgctaaacgtaccctgtgggttggtcagacctctggttacaactctacctcttacgacgctaaaatcacccagttcttcctggaaggtgcttacacccaactcaacaacgacaaatactctatcgaaccgtacgctggtttcaactggctccatgtttctaccgactctatcaacgaacaggttggtaacatgcagttctctaccaaaaccgacaaccaggacatccaggttggcactatcggtctgcgtggtggttacccgttcaccgctggttctatcaacatggctctgaaaggtgacgtttctgcttctcacctgttcggtgacaaccgtccggaagctcaactctacctgtctaactctggtgaagctaccctgcgtggtggtaaactggacaacctgttcggtgttggtctgggtgttgacgctcaactcaacaaatctaccaccttcggtgtttcttaccagggtcagtacaactctgacgtttcttcttctggtatcaacgctaccctgaaagttaacttcg BBa_K197034_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagagtcttccgaggaatacaaaggtggttactacccgggtaacacctaccactaccactctggtggttcttaccacggttctggttaccacggtggttacaaaggtaaatactacggtaaagctaaaaaatactactacaaatacaaaaactctggtaaatacaaatacctgaaaaaagctcgtaaataccaccgtaaaggttacaaaaaatactacggtggtggttcttcttactagagagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactataagacacgtgctgaagtcaagtttgaaggtgatacacttgttaatagaatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaagttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtccaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgcagcaaacgacgaaaactacgctttagtagcttactagaggatctgaaaactctaacgctcgtatgtggatgactggcgttggtcagtggggtaacctggactacggttactctaacatggacgttgacttctacgctggtctggttggtgctgaaatcgacgttaccgaaaacaccaaagctggtctgttcttcggtgctggttctacccactacaaagctcacgaatacggtaaaatcgactctaacgacctccatgttggtctgtacggtgtttctaacatcaaagacgttgctgctgttaactacggtatcacccacaccaaccaggaccgtgacgctaaacgtaccctgtgggttggtcagacctctggttacaactctacctcttacgacgctaaaatcacccagttcttcctggaaggtgcttacacccaactcaacaacgacaaatactctatcgaaccgtacgctggtttcaactggctccatgtttctaccgactctatcaacgaacaggttggtaacatgcagttctctaccaaaaccgacaaccaggacatccaggttggcactatcggtctgcgtggtggttacccgttcaccgctggttctatcaacatggctctgaaaggtgacgtttctgcttctcacctgttcggtgacaaccgtccggaagctcaactctacctgtctaactctggtgaagctaccctgcgtggtggtaaactggacaacctgttcggtgttggtctgggtgttgacgctcaactcaacaaatctaccaccttcggtgtttcttaccagggtcagtacaactctgacgtttcttcttctggtatcaacgctaccctgaaagttaacttcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K197018_sequence 1 tcttccgaggaatacaaaggtggttactacccgggtaacacctaccactaccactctggtggttcttaccacggttctggttaccacggtggttacaaaggtaaatactacggtaaagctaaaaaatactactacaaatacaaaaactctggtaaatacaaatacctgaaaaaagctcgtaaataccaccgtaaaggttacaaaaaatactacggtggtggttcttct BBa_K197030_sequence 1 agtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactataagacacgtgctgaagtcaagtttgaaggtgatacacttgttaatagaatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaagttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtccaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgcagcaaacgacgaaaactacgctttagtagct BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M10093_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z