LOCUS BBa_K197036 2580 bp DNA linear 25-Nov-2024 DEFINITION {Pbad.rbs.prepro.StrepTag}{KILR}{eCPX}(dblterm) ACCESSION BBa_K197036 VERSION BBa_K197036.1 FEATURES Location/Qualifiers CDS 50..805 /label=AraC promoter 956..1193 /label=Pbad CDS 1290..1358 /label=PrePro Sequence CDS 1374..1412 /label=Streptavidin Binding Peptide RBS 1274..1289 /label=rbs misc_feature 1..1452 /label=BBa_M10093 CDS 1461..1571 /label=BBa_K197022 CDS 1580..2443 /label=BBa_K197016 stem_loop 2463..2506 /label=stem_loop misc_feature 2452..2531 /label=BBa_B0010 terminator 2452..2531 /label=BBa_B0010 stem_loop 2547..2566 /label=T7 TE misc_feature 2573..2573 /label=stop polyA_site 2567..2580 /label=polya misc_feature 2540..2580 /label=BBa_B0012 terminator 2540..2580 /label=BBa_B0012 ORIGIN 1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac 301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc 481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga 1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc 1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca 1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac 1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg 1441 cagtctgggc agtactagag gatctgttaa agaactggaa gacaaaaacg aagaactgct 1501 gtctaaaatc taccacctgc gtaacgaagt tgctcgtctg aaaaaactgg ttggtgaacg 1561 tggtggttgc gtactagagg atctggtaaa cgtggaacgt ggtttgatcg cccggagctg 1621 gaggtgcatt atcagttcct cgaaaacgat gatttcagtt tcggcctgac cggcggtttc 1681 cgtaattatg gttatcacta cgttgatgaa ccgggtaaag acacggcgaa tatgcagcgc 1741 tggaaaatcg cgccagactg ggatgtgaaa ctgactgacg atttacgttt caacggttgg 1801 ttgtcgatgt ataaatttgc caacgatctg aacactaccg gttacgctga tacccgtgtc 1861 gaaacggaaa caggtctgca atataccttc aacgaaacgg ttgccttgcg agtgaactat 1921 tatcttgagc gcggcttcaa tatggacgac agccgcaata acggtgagtt ttccacgcaa 1981 gaaattcgcg tctatttgcc gctgacgctc ggcaaccact cggtgacgcc gtatacgcgc 2041 attgggctgg atcgctggag taactgggac tggcaggatg atattgaacg tgaaggccat 2101 gattttaacc gtgtaggttt attttacggt tatgatttcc agaacggact ttccgtttcg 2161 ctggaatacg cgtttgagtg gcaggatcac gacgaaggcg acagtgataa attccattat 2221 gcaggtgtcg gcgtaaatta ctcgttagga tctaagtctc gtcgcgagga aaggaacgac 2281 tggcacttta atatcggcgc gatgtacgaa atagaaaacg tcgagggtta tggcgaagat 2341 atggatgggc tggcggagcc ttcagtctat tttaatgccg ccaacgggcc gtggagaatt 2401 gctctggcct attatcggga agggccggta gattatagcg cggtactaga gccaggcatc 2461 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2521 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //