LOCUS       BBa_K197037             3000 bp    DNA     linear       22-Nov-2024
DEFINITION  {Pbad.rbs.prepro.StrepTag}{AG4}{espP(beta)!}{dblTerm}
ACCESSION   BBa_K197037
VERSION     BBa_K197037.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     CDS             1461..1502
                     /label=BBa_K197023
     CDS             1511..2863
                     /label=BBa_K197009
     stem_loop       2883..2926
                     /label=stem_loop
     misc_feature    2872..2951
                     /label=BBa_B0010
     terminator      2872..2951
                     /label=BBa_B0010
     stem_loop       2967..2986
                     /label=T7 TE
     misc_feature    2993..2993
                     /label=stop
     polyA_site      2987..3000
                     /label=polya
     misc_feature    2960..3000
                     /label=BBa_B0012
     terminator      2960..3000
                     /label=BBa_B0012
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagag gatctaaccc gtcttctctg ttccgttacc tgccgtctga
     1501 cgtactagag gatctgcccc gtcagcatct gccactatga acaacacctg gtggcaacta
     1561 accggagatt ctgcgctgaa aacactgaaa agtacaaaca gcatggtcta tttcactgac
     1621 agcgcaaaca ataagaaatt ccatacgctg acggtcgatg agctggcaac cagcaacagc
     1681 gcctatgcga tgcgtacaaa cctttctgaa tcagacaaac tggaggtcaa aaaacacttg
     1741 tctggtgaga acaatatttt actcgttgat ttccttcaga aaccaacgcc tgaaaaacaa
     1801 ctgaatattg aactggtaag cgcgccaaaa gacaccaatg aaaatgtctt taaagccagt
     1861 aaacaaacca ttggtttcag tgatgtaacg ccggtcatta caaccaggga aaccgatgac
     1921 aaaataacat ggtcactgac aggctataac acggtagcaa acaaggaagc aacccggaat
     1981 gccgccgccc tgttctctgt tgactataaa gcgtttctga acgaggtcaa caacctgaac
     2041 aaacgtatgg gtgacctgcg tgatatcaac ggcgaagccg gtgcatgggc acgcatcatg
     2101 agcggtaccg gctctgccag tggtggtttc agtgacaact acacgcacgt tcaggtcggg
     2161 gtcgacaaaa aacacgagct ggacggactg gatttgttta ccggtttcac tgtcacacac
     2221 actgacagca gtgcctccgc cgatgttttc agtggtaaaa cgaagtctgt gggggctggc
     2281 ctgtatgctt ccgccatgtt tgattccggt gcctatatcg acctgattgg caagtatgtt
     2341 caccatgata atgagtacac tgcaaccttt gccggactcg gaacccgtga ttacagcacg
     2401 cattcatggt atgccggtgc agaagcgggc taccgctatc atgtcactga ggatgcctgg
     2461 attgagccac aggctgagct ggtttacggt tctgtatccg gtaaacagtt tgcatggaag
     2521 gaccagggaa tgcatctgtc catgaaggac aaggactaca atccgctgat tggccgaacg
     2581 ggtgtggatg tgggtaaatc cttctctggt aaggactgga aagtgacagc ccgtgccggt
     2641 ctgggctacc agttcgacct gctggctaac ggcgaaaccg tattgcggga tgcatctggt
     2701 gaaaaacgca tcaaaggtga aaaggacagc cgtatgctga tgtccgttgg cctgaatgca
     2761 gaaatcaggg ataacgtccg ctttggactg gagtttgaga aatccgcctt tggtaagtac
     2821 aacgttgata atgctgtcaa cgctaatttc cgttactcgt tcgtactaga gccaggcatc
     2881 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2941 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//