BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_M10093 1 BBa_M10093 {Pbad.rbs.prepro.StrepTag&#707;} 2009-04-26T11:00:00Z 2015-05-08T01:13:51Z PCR from synthesized DNAs Composite part with Arabinose promoter, ribosome binding site, prepro periplasmic targetting sequence, and a streptavidin-binding tag in frame with the BglBricks scar. false false _271_ 0 483 271 Not in stock false N/A false John Anderson annotation2002589 1 AraC range2002589 1 50 805 annotation2002591 1 PrePro Sequence range2002591 1 1290 1358 annotation2002592 1 Streptavidin Binding Peptide range2002592 1 1374 1412 annotation2002590 1 Pbad range2002590 1 956 1193 annotation2002593 1 rbs range2002593 1 1274 1289 BBa_K197009 1 BBa_K197009 {espP(beta)} 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z From genomic source: Eschericia coli. A 12-stranded beta barrel. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein. false false _294_ 0 5247 9 It's complicated false N/A false Gabriela Guzman Lopez Aguado BBa_K197023 1 BBa_K197023 Ag4 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z Wobble Odvs002F/Odvs002R to make M10023 (Bdvs002) (67 bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10023 {<AG4>} (3222bp) ---- Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG The Silver Binding peptide binds and reduces silver for biomineralization purposes. false false _294_ 0 5247 9 It's complicated false N/A false Gabriela Guzman Lopez Aguado BBa_K197037 1 BBa_K197037 {Pbad.rbs.prepro.StrepTag}{AG4}{espP(beta)!}{dblTerm} 2009-10-20T11:00:00Z 2015-05-08T01:11:18Z BglBricks Assembly This is a cell surface display device that will enable engineered cells to bind silver via the fusion of a silver binding peptide to the surface of E.coli. false false _294_ 0 4745 271 It's complicated false N/A false Jennifer Brophy component2062210 1 BBa_B0010 component2062208 1 BBa_K197023 component2062207 1 BBa_M10093 component2062209 1 BBa_K197009 component2062212 1 BBa_B0012 annotation2062212 1 BBa_B0012 range2062212 1 2960 3000 annotation2062207 1 BBa_M10093 range2062207 1 1 1452 annotation2062208 1 BBa_K197023 range2062208 1 1461 1502 annotation2062210 1 BBa_B0010 range2062210 1 2872 2951 annotation2062209 1 BBa_K197009 range2062209 1 1511 2863 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K197037_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcagtactagaggatctaacccgtcttctctgttccgttacctgccgtctgacgtactagaggatctgccccgtcagcatctgccactatgaacaacacctggtggcaactaaccggagattctgcgctgaaaacactgaaaagtacaaacagcatggtctatttcactgacagcgcaaacaataagaaattccatacgctgacggtcgatgagctggcaaccagcaacagcgcctatgcgatgcgtacaaacctttctgaatcagacaaactggaggtcaaaaaacacttgtctggtgagaacaatattttactcgttgatttccttcagaaaccaacgcctgaaaaacaactgaatattgaactggtaagcgcgccaaaagacaccaatgaaaatgtctttaaagccagtaaacaaaccattggtttcagtgatgtaacgccggtcattacaaccagggaaaccgatgacaaaataacatggtcactgacaggctataacacggtagcaaacaaggaagcaacccggaatgccgccgccctgttctctgttgactataaagcgtttctgaacgaggtcaacaacctgaacaaacgtatgggtgacctgcgtgatatcaacggcgaagccggtgcatgggcacgcatcatgagcggtaccggctctgccagtggtggtttcagtgacaactacacgcacgttcaggtcggggtcgacaaaaaacacgagctggacggactggatttgtttaccggtttcactgtcacacacactgacagcagtgcctccgccgatgttttcagtggtaaaacgaagtctgtgggggctggcctgtatgcttccgccatgtttgattccggtgcctatatcgacctgattggcaagtatgttcaccatgataatgagtacactgcaacctttgccggactcggaacccgtgattacagcacgcattcatggtatgccggtgcagaagcgggctaccgctatcatgtcactgaggatgcctggattgagccacaggctgagctggtttacggttctgtatccggtaaacagtttgcatggaaggaccagggaatgcatctgtccatgaaggacaaggactacaatccgctgattggccgaacgggtgtggatgtgggtaaatccttctctggtaaggactggaaagtgacagcccgtgccggtctgggctaccagttcgacctgctggctaacggcgaaaccgtattgcgggatgcatctggtgaaaaacgcatcaaaggtgaaaaggacagccgtatgctgatgtccgttggcctgaatgcagaaatcagggataacgtccgctttggactggagtttgagaaatccgcctttggtaagtacaacgttgataatgctgtcaacgctaatttccgttactcgttcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K197023_sequence 1 gatctaacccgtcttctctgttccgttacctgccgtctgacg BBa_K197009_sequence 1 gatctgccccgtcagcatctgccactatgaacaacacctggtggcaactaaccggagattctgcgctgaaaacactgaaaagtacaaacagcatggtctatttcactgacagcgcaaacaataagaaattccatacgctgacggtcgatgagctggcaaccagcaacagcgcctatgcgatgcgtacaaacctttctgaatcagacaaactggaggtcaaaaaacacttgtctggtgagaacaatattttactcgttgatttccttcagaaaccaacgcctgaaaaacaactgaatattgaactggtaagcgcgccaaaagacaccaatgaaaatgtctttaaagccagtaaacaaaccattggtttcagtgatgtaacgccggtcattacaaccagggaaaccgatgacaaaataacatggtcactgacaggctataacacggtagcaaacaaggaagcaacccggaatgccgccgccctgttctctgttgactataaagcgtttctgaacgaggtcaacaacctgaacaaacgtatgggtgacctgcgtgatatcaacggcgaagccggtgcatgggcacgcatcatgagcggtaccggctctgccagtggtggtttcagtgacaactacacgcacgttcaggtcggggtcgacaaaaaacacgagctggacggactggatttgtttaccggtttcactgtcacacacactgacagcagtgcctccgccgatgttttcagtggtaaaacgaagtctgtgggggctggcctgtatgcttccgccatgtttgattccggtgcctatatcgacctgattggcaagtatgttcaccatgataatgagtacactgcaacctttgccggactcggaacccgtgattacagcacgcattcatggtatgccggtgcagaagcgggctaccgctatcatgtcactgaggatgcctggattgagccacaggctgagctggtttacggttctgtatccggtaaacagtttgcatggaaggaccagggaatgcatctgtccatgaaggacaaggactacaatccgctgattggccgaacgggtgtggatgtgggtaaatccttctctggtaaggactggaaagtgacagcccgtgccggtctgggctaccagttcgacctgctggctaacggcgaaaccgtattgcgggatgcatctggtgaaaaacgcatcaaaggtgaaaaggacagccgtatgctgatgtccgttggcctgaatgcagaaatcagggataacgtccgctttggactggagtttgagaaatccgcctttggtaagtacaacgttgataatgctgtcaacgctaatttccgttactcgttcg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M10093_sequence 1 ctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctttgggctagcgcattcgagctcggtacctttgaggtggttatgaaaaaaattgcatgtctttcagcactggccgcagttctggctttcaccgcaggtacttccgtagctggccagtctggccagtgggtgtgccacccgatgtgggaggtgatgtgcctgaggggagggtctgggtggaggggcatggagggagggcagtctgggcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z