LOCUS       BBa_K1971000            3939 bp    DNA     linear       26-Nov-2024
DEFINITION  psb1c3-ompt-crm197
ACCESSION   BBa_K1971000
VERSION     BBa_K1971000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2085
                     /label=BBa_K1971011
     promoter        2094..2112
                     /label=T7 promoter
     misc_feature    2113..2137
                     /label=lac operator
     RBS             2152..2174
                     /label=RBS
     CDS             2255..3871
                     /label=CRM197
     misc_feature    2187..2254
                     /label=SP-ompt
     misc_feature    3892..3939
                     /label=T7 terminator
     misc_feature    2094..3939
                     /label=BBa_K1971012
ORIGIN
        1 aggtctctac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
       61 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     1081 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1141 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     1201 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1261 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     1321 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     1381 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1441 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1501 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1561 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     1621 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1681 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1741 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1801 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1861 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1921 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1981 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     2041 taaggatgat ttctggaatt cgcggccgct tctagaggct gaggctacta gagtaatacg
     2101 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
     2161 ctttaagaag gagatatacc atgggcagca gcatgagggc gaaactgctg ggtatcgttc
     2221 tcaccacccc gatagcgata agttctttcg ctatgggagc tgatgatgtg gttgattcca
     2281 gcaaatcatt tgtgatggaa aatttcagca gctatcatgg caccaagccg ggctatgtgg
     2341 attccattca gaaaggcatc cagaaaccga agagcggcac ccagggcaat tatgacgatg
     2401 attggaagga gttctatagc accgataata agtatgacgc agcagggtat agcgtggaca
     2461 atgaaaatcc gctgagcggt aaagcgggtg gcgtagtgaa agtgacctat ccgggcctga
     2521 ccaaggtgct ggcgttaaaa gtggataacg cggaaaccat caagaaagaa ctgggcctgt
     2581 cactgaccga accgcttatg gagcaagtgg gcactgagga atttatcaag cgttttggcg
     2641 acggcgcgag ccgcgtggtg ttgtctcttc cgttcgctga aggtagcagc agtgtggaat
     2701 acattaacaa ctgggaacag gccaaagccc tgagcgttga gcttgaaatc aactttgaaa
     2761 cccgtggcaa acggggccag gatgcgatgt atgaatatat ggcgcaggcg tgtgcgggca
     2821 atcgcgtgcg tcgttccgtg ggaagttccc tgagttgcat aaatcttgat tgggatgtca
     2881 ttcgtgataa aacaaaaacc aaaattgaga gcctgaagga acatggaccc ataaagaaca
     2941 aaatgagtga atctccgaac aagaccgtgt cagaagaaaa agctaaacag tacctggagg
     3001 agtttcatca aaccgctctg gaacatccgg aactttcgga actgaaaacc gtgaccggca
     3061 caaatccggt gtttgcgggt gcgaattacg ccgcatgggc ggtgaatgtc gcgcaggtca
     3121 ttgatagcga aaccgccgat aacttagaga aaaccacggc ggctctgtcg attctgccgg
     3181 gaattggcag cgtgatgggt attgcggatg gcgcggttca ccataacacc gaggagattg
     3241 tggcacagag catcgcgctg tcctcgttaa tggtggccca agcaattccg ctggtgggcg
     3301 aactggtgga tatcggcttt gcggcttata actttgtcga aagcatcatt aacctgtttc
     3361 aggtggtgca taacagctac aatcgtccgg cgtattcacc cggacacaaa acacaaccgt
     3421 ttctgcatga cggctatgcg gtgtcttgga acaccgtgga ggattctatt atacgtaccg
     3481 gctttcaggg cgagtcgggc cacgatataa agatcacagc ggagaatacc ccgcttccga
     3541 ttgcgggggt cctgttgccg accattccgg gaaaacttga cgtgaataag agcaaaaccc
     3601 atattagcgt gaatggccgt aaaattcgta tgcgttgccg tgcgatagat ggtgacgtaa
     3661 ctttttgccg tccgaaatca cctgtgtatg tcggaaatgg agtgcatgcg aatcttcatg
     3721 tcgccttcca ccgttcgagc agcgaaaaga tccattctaa tgaaattagc tccgacagca
     3781 ttggtgtatt aggctatcag aaaacagtgg atcataccaa agtgaatagc aaactgagcc
     3841 tgttttttga gattaagagc taagctgagg cctgccaccg ctgagcaata actagcataa
     3901 ccccttgggg cctctaaacg ggtcttgagg ggttttttg
//