BBa_K1971012 1 BBa_K1971012 t7-ompt-crm197-t7 terminator 2016-10-19T11:00:00Z 2016-10-20T04:27:26Z pcr product from pet28a-ompt-crm197 t7-ompt-crm197 false false _2438_ 29894 29894 9 false none false KE GUAN YU annotation2525138 1 CRM197 range2525138 1 162 1778 annotation2525137 1 RBS range2525137 1 59 81 annotation2525139 1 SP-ompt range2525139 1 94 161 annotation2525140 1 T7 terminator range2525140 1 1799 1846 annotation2525135 1 T7 promoter range2525135 1 1 19 annotation2525136 1 lac operator range2525136 1 20 44 BBa_K1971011 1 BBa_K1971011 psb1c3 backbone with BsaI site 2016-10-19T11:00:00Z 2016-10-20T02:44:34Z psb1c3 circulized plasmid psb1c3 backbone with BsaI site false false _2438_ 29894 29894 9 false None false KE GUAN YU BBa_K1971000 1 BBa_K1971000 psb1c3-ompt-crm197 2016-10-13T11:00:00Z 2016-10-20T02:56:21Z all from artificial sequence. this is the PSB1C3 plasmid with the expression cassette from PET28a with gene for CRM197, a nontoxic variant of diphtheria toxin, connect with signal peptile ompt, a signal peptide for secretion. false false _2438_ 29894 29894 9 false the constuction of PET28a-ompt-CRM197 is tough false KE GUAN YU component2525074 1 BBa_K1971011 component2525075 1 BBa_K1971012 annotation2525074 1 BBa_K1971011 range2525074 1 1 2085 annotation2525075 1 BBa_K1971012 range2525075 1 2094 3939 BBa_K1971011_sequence 1 aggtctctactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagaggctgaggc BBa_K1971000_sequence 1 aggtctctactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagaggctgaggctactagagtaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagcatgagggcgaaactgctgggtatcgttctcaccaccccgatagcgataagttctttcgctatgggagctgatgatgtggttgattccagcaaatcatttgtgatggaaaatttcagcagctatcatggcaccaagccgggctatgtggattccattcagaaaggcatccagaaaccgaagagcggcacccagggcaattatgacgatgattggaaggagttctatagcaccgataataagtatgacgcagcagggtatagcgtggacaatgaaaatccgctgagcggtaaagcgggtggcgtagtgaaagtgacctatccgggcctgaccaaggtgctggcgttaaaagtggataacgcggaaaccatcaagaaagaactgggcctgtcactgaccgaaccgcttatggagcaagtgggcactgaggaatttatcaagcgttttggcgacggcgcgagccgcgtggtgttgtctcttccgttcgctgaaggtagcagcagtgtggaatacattaacaactgggaacaggccaaagccctgagcgttgagcttgaaatcaactttgaaacccgtggcaaacggggccaggatgcgatgtatgaatatatggcgcaggcgtgtgcgggcaatcgcgtgcgtcgttccgtgggaagttccctgagttgcataaatcttgattgggatgtcattcgtgataaaacaaaaaccaaaattgagagcctgaaggaacatggacccataaagaacaaaatgagtgaatctccgaacaagaccgtgtcagaagaaaaagctaaacagtacctggaggagtttcatcaaaccgctctggaacatccggaactttcggaactgaaaaccgtgaccggcacaaatccggtgtttgcgggtgcgaattacgccgcatgggcggtgaatgtcgcgcaggtcattgatagcgaaaccgccgataacttagagaaaaccacggcggctctgtcgattctgccgggaattggcagcgtgatgggtattgcggatggcgcggttcaccataacaccgaggagattgtggcacagagcatcgcgctgtcctcgttaatggtggcccaagcaattccgctggtgggcgaactggtggatatcggctttgcggcttataactttgtcgaaagcatcattaacctgtttcaggtggtgcataacagctacaatcgtccggcgtattcacccggacacaaaacacaaccgtttctgcatgacggctatgcggtgtcttggaacaccgtggaggattctattatacgtaccggctttcagggcgagtcgggccacgatataaagatcacagcggagaataccccgcttccgattgcgggggtcctgttgccgaccattccgggaaaacttgacgtgaataagagcaaaacccatattagcgtgaatggccgtaaaattcgtatgcgttgccgtgcgatagatggtgacgtaactttttgccgtccgaaatcacctgtgtatgtcggaaatggagtgcatgcgaatcttcatgtcgccttccaccgttcgagcagcgaaaagatccattctaatgaaattagctccgacagcattggtgtattaggctatcagaaaacagtggatcataccaaagtgaatagcaaactgagcctgttttttgagattaagagctaagctgaggcctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K1971012_sequence 1 taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaagaaggagatataccatgggcagcagcatgagggcgaaactgctgggtatcgttctcaccaccccgatagcgataagttctttcgctatgggagctgatgatgtggttgattccagcaaatcatttgtgatggaaaatttcagcagctatcatggcaccaagccgggctatgtggattccattcagaaaggcatccagaaaccgaagagcggcacccagggcaattatgacgatgattggaaggagttctatagcaccgataataagtatgacgcagcagggtatagcgtggacaatgaaaatccgctgagcggtaaagcgggtggcgtagtgaaagtgacctatccgggcctgaccaaggtgctggcgttaaaagtggataacgcggaaaccatcaagaaagaactgggcctgtcactgaccgaaccgcttatggagcaagtgggcactgaggaatttatcaagcgttttggcgacggcgcgagccgcgtggtgttgtctcttccgttcgctgaaggtagcagcagtgtggaatacattaacaactgggaacaggccaaagccctgagcgttgagcttgaaatcaactttgaaacccgtggcaaacggggccaggatgcgatgtatgaatatatggcgcaggcgtgtgcgggcaatcgcgtgcgtcgttccgtgggaagttccctgagttgcataaatcttgattgggatgtcattcgtgataaaacaaaaaccaaaattgagagcctgaaggaacatggacccataaagaacaaaatgagtgaatctccgaacaagaccgtgtcagaagaaaaagctaaacagtacctggaggagtttcatcaaaccgctctggaacatccggaactttcggaactgaaaaccgtgaccggcacaaatccggtgtttgcgggtgcgaattacgccgcatgggcggtgaatgtcgcgcaggtcattgatagcgaaaccgccgataacttagagaaaaccacggcggctctgtcgattctgccgggaattggcagcgtgatgggtattgcggatggcgcggttcaccataacaccgaggagattgtggcacagagcatcgcgctgtcctcgttaatggtggcccaagcaattccgctggtgggcgaactggtggatatcggctttgcggcttataactttgtcgaaagcatcattaacctgtttcaggtggtgcataacagctacaatcgtccggcgtattcacccggacacaaaacacaaccgtttctgcatgacggctatgcggtgtcttggaacaccgtggaggattctattatacgtaccggctttcagggcgagtcgggccacgatataaagatcacagcggagaataccccgcttccgattgcgggggtcctgttgccgaccattccgggaaaacttgacgtgaataagagcaaaacccatattagcgtgaatggccgtaaaattcgtatgcgttgccgtgcgatagatggtgacgtaactttttgccgtccgaaatcacctgtgtatgtcggaaatggagtgcatgcgaatcttcatgtcgccttccaccgttcgagcagcgaaaagatccattctaatgaaattagctccgacagcattggtgtattaggctatcagaaaacagtggatcataccaaagtgaatagcaaactgagcctgttttttgagattaagagctaagctgaggcctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z