LOCUS BBa_K1971005 5189 bp DNA linear 24-Nov-2024 DEFINITION pet28a backbone with BsaI site ACCESSION BBa_K1971005 VERSION BBa_K1971005.1 FEATURES Location/Qualifiers ORIGIN 1 ggtctcactg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 61 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct 121 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 181 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 241 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 301 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 361 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 421 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 481 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 541 ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa 601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct 661 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata 721 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat 781 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct 841 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact 901 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag 961 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc 1021 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa 1081 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat 1141 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca 1201 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt 1261 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac 1321 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca 1381 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc 1441 ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg 1501 taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 1561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 1621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 1681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 1741 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 1801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 1861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 1921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 1981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 2041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 2101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 2161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 2221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 2281 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 2341 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct 2401 gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca 2461 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac 2521 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 2581 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 2641 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt 2701 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc 2761 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt 2821 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga 2881 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg 2941 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta 3001 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca 3061 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga 3121 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct 3181 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc 3241 tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct 3301 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca 3361 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct 3421 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga 3481 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt 3541 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat 3601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 3661 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt 3721 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt 3781 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt 3841 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc 3901 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg 3961 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga 4021 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga 4081 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc 4141 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta 4201 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat 4261 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga 4321 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag 4381 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg 4441 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca 4501 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa 4561 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc 4621 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat 4681 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc 4741 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg 4801 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc 4861 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac 4921 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc 4981 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac 5041 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata 5101 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 5161 gagatatacc atgggcagca gctgagacc //