LOCUS       BBa_K1971012            1846 bp    DNA     linear       22-Nov-2024
DEFINITION  t7-ompt-crm197-t7 terminator
ACCESSION   BBa_K1971012
VERSION     BBa_K1971012.1  
FEATURES             Location/Qualifiers
     promoter        1..19
                     /label=T7 promoter
     misc_feature    20..44
                     /label=lac operator
     RBS             59..81
                     /label=RBS
     CDS             162..1778
                     /label=CRM197
     misc_feature    94..161
                     /label=SP-ompt
     misc_feature    1799..1846
                     /label=T7 terminator
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagca tgagggcgaa actgctgggt
      121 atcgttctca ccaccccgat agcgataagt tctttcgcta tgggagctga tgatgtggtt
      181 gattccagca aatcatttgt gatggaaaat ttcagcagct atcatggcac caagccgggc
      241 tatgtggatt ccattcagaa aggcatccag aaaccgaaga gcggcaccca gggcaattat
      301 gacgatgatt ggaaggagtt ctatagcacc gataataagt atgacgcagc agggtatagc
      361 gtggacaatg aaaatccgct gagcggtaaa gcgggtggcg tagtgaaagt gacctatccg
      421 ggcctgacca aggtgctggc gttaaaagtg gataacgcgg aaaccatcaa gaaagaactg
      481 ggcctgtcac tgaccgaacc gcttatggag caagtgggca ctgaggaatt tatcaagcgt
      541 tttggcgacg gcgcgagccg cgtggtgttg tctcttccgt tcgctgaagg tagcagcagt
      601 gtggaataca ttaacaactg ggaacaggcc aaagccctga gcgttgagct tgaaatcaac
      661 tttgaaaccc gtggcaaacg gggccaggat gcgatgtatg aatatatggc gcaggcgtgt
      721 gcgggcaatc gcgtgcgtcg ttccgtggga agttccctga gttgcataaa tcttgattgg
      781 gatgtcattc gtgataaaac aaaaaccaaa attgagagcc tgaaggaaca tggacccata
      841 aagaacaaaa tgagtgaatc tccgaacaag accgtgtcag aagaaaaagc taaacagtac
      901 ctggaggagt ttcatcaaac cgctctggaa catccggaac tttcggaact gaaaaccgtg
      961 accggcacaa atccggtgtt tgcgggtgcg aattacgccg catgggcggt gaatgtcgcg
     1021 caggtcattg atagcgaaac cgccgataac ttagagaaaa ccacggcggc tctgtcgatt
     1081 ctgccgggaa ttggcagcgt gatgggtatt gcggatggcg cggttcacca taacaccgag
     1141 gagattgtgg cacagagcat cgcgctgtcc tcgttaatgg tggcccaagc aattccgctg
     1201 gtgggcgaac tggtggatat cggctttgcg gcttataact ttgtcgaaag catcattaac
     1261 ctgtttcagg tggtgcataa cagctacaat cgtccggcgt attcacccgg acacaaaaca
     1321 caaccgtttc tgcatgacgg ctatgcggtg tcttggaaca ccgtggagga ttctattata
     1381 cgtaccggct ttcagggcga gtcgggccac gatataaaga tcacagcgga gaataccccg
     1441 cttccgattg cgggggtcct gttgccgacc attccgggaa aacttgacgt gaataagagc
     1501 aaaacccata ttagcgtgaa tggccgtaaa attcgtatgc gttgccgtgc gatagatggt
     1561 gacgtaactt tttgccgtcc gaaatcacct gtgtatgtcg gaaatggagt gcatgcgaat
     1621 cttcatgtcg ccttccaccg ttcgagcagc gaaaagatcc attctaatga aattagctcc
     1681 gacagcattg gtgtattagg ctatcagaaa acagtggatc ataccaaagt gaatagcaaa
     1741 ctgagcctgt tttttgagat taagagctaa gctgaggcct gccaccgctg agcaataact
     1801 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttg
//