BBa_K1972004 1 BBa_K1972004 dszA (a monooxygenase gene ), E.coli-optimized 2016-10-12T11:00:00Z 2016-10-13T08:03:40Z This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1) DszA is a monoxygenase which can convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszA require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working. This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1) false false _2439_ 29948 29948 9 false No. false Shuli Liang annotation2500236 1 dszA-optimized range2500236 1 22 1383 annotation2500235 1 RBS range2500235 1 1 21 BBa_K1972004_sequence 1 caactgcaaggacaaccttcaatgacccagcagcgccagatgcaccttgcaggctttttcagcgcaggcaatgtgacccatgcacatggtgcatggcgtcataccgatgcctctaatgattttctgagcggtaaatattatcagcatattgcacgtaccttagaacgcggcaaatttgatctgctgtttctgccggatggtttagccgttgaagatagctatggagacaatctggataccggcgtgggcttaggtggtcagggtgcagttgccttagaaccggcaagcgttgtggcaaccatggccgcagtgaccgaacacctgggtttaggtgcaaccattagcgccacctattatccgccgtatcatgtggcacgtgtgtttgccaccttagatcagctgagcggcggtcgtgtgtcttggaatgttgtgacctcactgaatgatgcagaagcacgtaattttggtattaatcagcatctggaacatgatgcacgctatgatcgcgccgatgaatttctggaagccgtgaaaaaactgtggaactcttgggatgaagatgccctggtgctggataaagcagccggcgtgtttgccgatccggccaaagttcattatgttgatcatcatggcgaatggttaaatgtgcgcggcccgttacaggttccgcgtagtccgcagggcgaaccggttattttacaggcaggcctgtctccgcgcggtcgtcgctttgccggtaaatgggcagaagcagtgttttcactggccccgaatctggaagttatgcaggcaacctatcagggtattaaagcagaagttgatgccgcaggtcgcgatccggatcagaccaaaatttttaccgccgttatgccggtgctgggcgaaagtcaggcagtggcccaggaacgcttagaatatttgaactcactggttcatccggaagttggcctgtctaccctgagtagtcataccggtattaatctggcagcctatccgttagataccccgattaaagatattctgcgcgacttgcaggatcgtaatgttccgacccagctgcatatgtttgcagccgccacccatagtgaagaactgaccttagccgaaatgggtcgtcgctatggtaccaatgtgggctttgttccgcagtgggccggtaccggcgaacagattgccgatgaactgattcgtcattttgaaggcggcgccgcagatggctttattatttcaccggcctttctgccgggtagctatgatgaatttgtggatcaggttgttccggtgttacaggatcgtggctattttcgcaccgaatatcagggtaataccctgcgtgatcatctgggcttacgcgttccgcagttacagggtcagccgagctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z