LOCUS       BBa_K1972008            1307 bp    DNA     linear       07-Dec-2025
DEFINITION  dszB with tac promoter induced by IPTG
ACCESSION   BBa_K1972008
VERSION     BBa_K1972008.1  
FEATURES             Location/Qualifiers
     promoter        1..53
                     /label=tac promoter
     RBS             54..72
                     /label=RBS
     CDS             73..1170
                     /label=dszB-optimized
     CDS             1..1170
                     /label=BBa_K1972005
     stem_loop       1190..1233
                     /label=stem_loop
     misc_feature    1179..1258
                     /label=BBa_B0010
     terminator      1179..1258
                     /label=BBa_B0010
     stem_loop       1274..1293
                     /label=T7 TE
     misc_feature    1300..1300
                     /label=stop
     polyA_site      1294..1307
                     /label=polya
     misc_feature    1267..1307
                     /label=BBa_B0012
     terminator      1267..1307
                     /label=BBa_B0012
     terminator      1179..1307
                     /label=BBa_B0015
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caaaactgca
       61 aggacaacct tcatgacctc tcgcgttgat ccggccaatc cgggtagcga actggatagt
      121 gccattcgcg ataccttaac ctatagtaat tgtccggttc cgaatgcact gttaaccgcc
      181 tctgaaagcg gctttctgga tgcagcaggt attgaactgg atgtgctgag cggccagcag
      241 ggtaccgttc attttaccta tgatcagccg gcatataccc gctttggcgg tgaaattccg
      301 ccgctgctga gtgaaggcct gcgtgcccct ggtcgtaccc gcttactggg tattaccccg
      361 ttactgggtc gccagggctt cttcgtgcgc gatgatagtc cgattaccgc agccgcagac
      421 ctcgcaggtc gtcgtattgg tgtgagcgca tcagccattc gtattctgcg cggtcagctc
      481 ggcgactatc tggaattaga tccgtggcgt cagaccttag ttgcattagg ctcatgggaa
      541 gcacgtgcct tattacatac cctggaacat ggcgaattag gcgttgatga tgttgaactg
      601 gttccgattt cttcaccggg tgtggatgtt ccggccgaac agttagaaga aagtgcaacc
      661 gttaaaggtg ccgatctgtt tccggatgtt gcacgcggtc aggcagccgt gctggcaagt
      721 ggcgatgtgg atgccctgta ttcttggctg ccgtgggcag gcgaattaca ggcaaccggc
      781 gcacgtccgg ttgtggacct cggtttagat gaacgtaatg catatgcaag tgtttggacc
      841 gtgtctagcg gtttagttcg ccagagacct ggcctggttc agcgtctggt tgatgccgcc
      901 gttgatgcag gcttatgggc acgcgatcat tcagatgccg tgacctcact gcatgccgca
      961 aatctgggtg tgtctaccgg cgcagttggt cagggctttg gcgcagattt tcagcagcgt
     1021 ctggttccgc gtttagatca tgatgcctta gccctgttag aacgcaccca gcagtttctg
     1081 ctgaccaaca acctgttaca ggaaccggtg gccttagatc agtgggccgc cccggaattt
     1141 ttaaacaaca gcttaaatcg tcatcgctaa tactagagcc aggcatcaaa taaaacgaaa
     1201 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1261 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//